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Title

SARS-CoV-Encoded Small RNAs Contribute to Infection-Associated Lung Pathology

AuthorsMorales, Lucía CSIC ORCID ; Oliveros, Juan C.; Fernandez-Delgado, Raúl ; tenOever, Benjamin Robert; Enjuanes Sánchez, Luis CSIC ORCID ; Solá Gurpegui, Isabel
KeywordsCoronavirus
SARS-CoV
Small viral RNAs
Virus-host interaction
Lung inflammatory pathology
Antagomirs
Deep sequencing
Non-coding RNAs
Innate immune response
Antiviral
Issue Date8-Mar-2017
PublisherCell Press
CitationCell Host and Microbe 21(3): 344-355 (2017)
AbstractSevere acute respiratory syndrome coronavirus (SARS-CoV) causes lethal disease in humans, which is characterized by exacerbated inflammatory response and extensive lung pathology. To address the relevance of small non-coding RNAs in SARS-CoV pathology, we deep sequenced RNAs from the lungs of infected mice and discovered three 18–22 nt small viral RNAs (svRNAs). The three svRNAs were derived from the nsp3 (svRNA-nsp3.1 and -nsp3.2) and N (svRNA-N) genomic regions of SARS-CoV. Biogenesis of CoV svRNAs was RNase III, cell type, and host species independent, but it was dependent on the extent of viral replication. Antagomir-mediated inhibition of svRNA-N significantly reduced in vivo lung pathology and pro-inflammatory cytokine expression. Taken together, these data indicate that svRNAs contribute to SARS-CoV pathogenesis and highlight the potential of svRNA-N antagomirs as antivirals.
Publisher version (URL)https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5662013/
URIhttp://hdl.handle.net/10261/204227
DOI10.1016/j.chom.2017.01.015
Identifiersdoi: 10.1016/j.chom.2017.01.015
e-issn: 1934-6069
pmid: 28216251
Appears in Collections:(CNB) Artículos
(VICYT) Colección Especial COVID-19

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