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Title

Immunization with an attenuated severe acute respiratory syndrome coronavirus deleted in E protein protects against lethal respiratory disease

AuthorsNetland, Jason; DeDiego, Marta L. CSIC ORCID ; Zhao, Jincun; Fett, Craig; Álvarez, Enrique ; Nieto-Torres, José L. ; Enjuanes Sánchez, Luis CSIC ORCID ; Perlman, Stanley
KeywordsSevere Acute Respiratory syndrome
Rodent model
Vaccine
Coronavirus
Respiratory virus
T cell response
Issue Date2010
PublisherAcademic Press
CitationVirology 399(1): 120-128 (2010)
AbstractThe severe acute respiratory syndrome coronavirus (SARS-CoV) caused substantial morbidity and mortality in 2002-2003. Deletion of the envelope (E) protein modestly diminished virus growth in tissue culture but abrogated virulence in animals. Here, we show that immunization with rSARS-CoV-ΔE or SARS-CoV-Δ[E,6-9b] (deleted in accessory proteins (6, 7a, 7b, 8a, 8b, 9b) in addition to E) nearly completely protected BALB/c mice from fatal respiratory disease caused by mouse-adapted SARS-CoV and partly protected hACE2 Tg mice from lethal disease. hACE2 Tg mice, which express the human SARS-CoV receptor, are extremely susceptible to infection. We also show that rSARS-CoV-ΔE and rSARS-CoV-Δ[E,6-9b] induced anti-virus T cell and antibody responses. Further, the E-deleted viruses were stable after 16 blind passages through tissue culture cells, with only a single mutation in the surface glycoprotein detected. The passaged virus remained avirulent in mice. These results suggest that rSARS-CoV-ΔE is an efficacious vaccine candidate that might be useful if SARS recurred. © 2009 Elsevier Inc. All rights reserved.
Publisher version (URL)https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830353
URIhttp://hdl.handle.net/10261/204187
DOI10.1016/j.virol.2010.01.004
Identifiersdoi: 10.1016/j.virol.2010.01.004
issn: 0042-6822
pmid: 20110095
Appears in Collections:(CNB) Artículos
(VICYT) Colección Especial COVID-19

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