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Title

Three-Dimensional Genomic Structure and Cohesin Occupancy Correlate with Transcriptional Activity during Spermatogenesis

AuthorsVara, Covadonga; Paytuví-Gallart, Andreu; Cuartero, Yasmina; Le Dily, Francois; Garcia, Francisca; Salvà-Castro, Judit; Gomez-H, Laura; Julià, Eva; Moutinho, Catia; Cigliano, Riccardo Aiese; Sanseverino, Walter; Fornas, Oscar; Pendás, Alberto M. ; Heyn, Holger; Waters, Paul D.; Marti-Renom, Marc A.; Ruiz-Herrera, Aurora
Issue DateJul-2019
PublisherElsevier
CitationCell Reports 28(2): 352-367.e9 (2019)
AbstractMammalian gametogenesis involves dramatic and tightly regulated chromatin remodeling, whose regulatory pathways remain largely unexplored. Here, we generate a comprehensive high-resolution structural and functional atlas of mouse spermatogenesis by combining in situ chromosome conformation capture sequencing (Hi-C), RNA sequencing (RNA-seq), and chromatin immunoprecipitation sequencing (ChIP-seq) of CCCTC-binding factor (CTCF) and meiotic cohesins, coupled with confocal and super-resolution microscopy. Spermatogonia presents well-defined compartment patterns and topological domains. However, chromosome occupancy and compartmentalization are highly re-arranged during prophase I, with cohesins bound to active promoters in DNA loops out of the chromosomal axes. Compartment patterns re-emerge in round spermatids, where cohesin occupancy correlates with transcriptional activity of key developmental genes. The compact sperm genome contains compartments with actively transcribed genes but no fine-scale topological domains, concomitant with the presence of protamines. Overall, we demonstrate how genome-wide cohesin occupancy and transcriptional activity is associated with three-dimensional (3D) remodeling during spermatogenesis, ultimately reprogramming the genome for the next generation.
Description© 2019 The Author(s).
Publisher version (URL)http://dx.doi.org/10.1016/j.celrep.2019.06.037
URIhttp://hdl.handle.net/10261/204021
DOIhttp://dx.doi.org/10.1016/j.celrep.2019.06.037
ISSN2211-1247
E-ISSN2211-1247
Appears in Collections:(IBMCC) Artículos
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