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Título: | Three-Dimensional Genomic Structure and Cohesin Occupancy Correlate with Transcriptional Activity during Spermatogenesis |
Autor: | Vara, Covadonga; Paytuví-Gallart, Andreu; Cuartero, Yasmina; Le Dily, Francois; García, Francisca; Salvà-Castro, Judit; Gómez Hernández, Laura CSIC; Julià, Eva; Moutinho, Catia; Cigliano, Riccardo Aiese; Sanseverino, Walter; Fornas, Oscar; Pendás, Alberto M. CSIC ORCID ; Heyn, Holger; Waters, Paul D.; Marti-Renom, Marc A.; Ruiz-Herrera, Aurora | Fecha de publicación: | jul-2019 | Editor: | Elsevier | Citación: | Cell Reports 28(2): 352-367.e9 (2019) | Resumen: | Mammalian gametogenesis involves dramatic and tightly regulated chromatin remodeling, whose regulatory pathways remain largely unexplored. Here, we generate a comprehensive high-resolution structural and functional atlas of mouse spermatogenesis by combining in situ chromosome conformation capture sequencing (Hi-C), RNA sequencing (RNA-seq), and chromatin immunoprecipitation sequencing (ChIP-seq) of CCCTC-binding factor (CTCF) and meiotic cohesins, coupled with confocal and super-resolution microscopy. Spermatogonia presents well-defined compartment patterns and topological domains. However, chromosome occupancy and compartmentalization are highly re-arranged during prophase I, with cohesins bound to active promoters in DNA loops out of the chromosomal axes. Compartment patterns re-emerge in round spermatids, where cohesin occupancy correlates with transcriptional activity of key developmental genes. The compact sperm genome contains compartments with actively transcribed genes but no fine-scale topological domains, concomitant with the presence of protamines. Overall, we demonstrate how genome-wide cohesin occupancy and transcriptional activity is associated with three-dimensional (3D) remodeling during spermatogenesis, ultimately reprogramming the genome for the next generation. | Descripción: | © 2019 The Author(s). | Versión del editor: | http://dx.doi.org/10.1016/j.celrep.2019.06.037 | URI: | http://hdl.handle.net/10261/204021 | DOI: | 10.1016/j.celrep.2019.06.037 | ISSN: | 2211-1247 | E-ISSN: | 2211-1247 |
Aparece en las colecciones: | (IBMCC) Artículos |
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