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Title

The mito-QC Reporter for Quantitative Mitophagy Assessment in Primary Retinal Ganglion Cells and Experimental Glaucoma Models

AuthorsRosignol, Ines; Villarejo-Zori, Beatriz CSIC ; Teresak, Petra ; Sierra-Filardi, Elena CSIC ORCID ; Pereiro, Xandra; Rodríguez-Muela, N. CSIC ORCID; Vecino, Elena; Vieira, Helena L. A.; Bell, Katharina; Boya, Patricia CSIC ORCID
KeywordsAutophagy
Retinal ganglion cells
Cell death
Primary neuronal cell culture
Mitophagy
Mito-QC reporter
Glaucoma
Issue Date2020
PublisherMultidisciplinary Digital Publishing Institute
CitationInternational Journal of Molecular Sciences 21(5): 1882 (2020)
AbstractMitochondrial damage plays a prominent role in glaucoma. The only way cells can degrade whole mitochondria is via autophagy, in a process called mitophagy. Thus, studying mitophagy in the context of glaucoma is essential to understand the disease. Up to date limited tools are available for analyzing mitophagy in vivo. We have taken advantage of the mito-QC reporter, a recently generated mouse model that allows an accurate mitophagy assessment to fill this gap. We used primary RGCs and retinal explants derived from mito-QC mice to quantify mitophagy activation in vitro and ex vivo. We also analyzed mitophagy in retinal ganglion cells (RGCs), in vivo, using different mitophagy inducers, as well as after optic nerve crush (ONC) in mice, a commonly used surgical procedure to model glaucoma. Using mito-QC reporter we quantified mitophagy induced by several known inducers in primary RGCs in vitro, ex vivo and in vivo. We also found that RGCs were rescued from some glaucoma relevant stress factors by incubation with the iron chelator deferiprone (DFP). Thus, the mito-QC reporter-based model is a valuable tool for accurately analyzing mitophagy in the context of glaucoma.
Description© 2020 by the authors.
Publisher version (URL)https://doi.org/10.3390/ijms21051882
URIhttp://hdl.handle.net/10261/203910
DOI10.3390/ijms21051882
E-ISSN1422-0067
Appears in Collections:(CIB) Artículos

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