English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/203876
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

TNF-α-induced oxidative stress and endothelial dysfunction in EA.hy926 cells is prevented by mate and green coffee extracts, 5-caffeoylquinic acid and its microbial metabolite, dihydrocaffeic acid

AuthorsWang, Shenli; Sarriá, Beatriz CSIC ORCID; Mateos, Raquel CSIC ORCID ; Goya, Luis CSIC ORCID; Bravo, Laura CSIC ORCID
KeywordsYerba mate
Green coffee
Microbial metabolites
Caffeoylquinic acids
Issue Date2019
PublisherTaylor & Francis
CitationInternational Journal of Food Sciences and Nutrition 70(3): 267-284 (2019)
AbstractThe main phenol in mate and coffee, 5-caffeoylquinic-acid (5-CQA), and its relevant microbial metabolites, dihydrocaffeic (DHCA) and dihydroferulic (DHFA) acids, have shown oxidative-stress protective effects in HepG2 cells. To evaluate possible endothelial-protective effects of the extracts and compounds, endothelial EA.hy926 cells were pre-treated with yerba mate (YME) and green coffee bean (GCBE) phenolic extracts, 5-CQA, DHCA and DHFA and afterwards stressed with tumour-necrosis-factor-alpha (TNF-α). Then oxidative-stress markers and endothelial-nitric-oxide-synthase levels were studied. TNF-α (10 ng/mL, 24 h) depleted reduced glutathione (GSH) and eNOS levels, increased reactive oxygen species (ROS) production, glutathione peroxidase (GPx) and reductase (GR) activities, and protein oxidation (carbonyl groups, CG) in EA.hy926 cells. Pre-treatment with YME, GCBE, 5-CQA, DHCA at certain physiological concentrations, lowered ROS production, recovered depleted GSH, reduced GR and GPx activities, and CG levels, and enhanced eNOS concentration.. YME, GCBE and 5-CQA show antioxidant effects in endothelial cells playing DHCA an important role in such protection; moreover, the extracts, 5-CQA, DHCA and DHFA increased eNOS levels.
Publisher version (URL)https://doi.org/10.1080/09637486.2018.1505834
URIhttp://hdl.handle.net/10261/203876
DOIhttp://dx.doi.org/10.1080/09637486.2018.1505834
ISSN0963-7486
E-ISSN1465-3478
Appears in Collections:(ICTAN) Artículos
Files in This Item:
File Description SizeFormat 
tnfacid.pdf510,12 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.