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Título

Generation of human androgenetic induced pluripotent stem cells

AutorChoi, Na Young; Bang, Jin Seok; Park, Yo Seph; Lee, Minseong; Hwang, Han Sung; Ko, Kisung; Myung, Soon Chul; Tapia, Natalia CSIC ORCID; Schöler, Hans R.; Kim, Gwang Jun; Ko, Kinarm
Fecha de publicación27-feb-2020
EditorNature Publishing Group
CitaciónScientific Reports 10(1):3614 (2020)
ResumenIn humans, parthenogenesis and androgenesis occur naturally in mature cystic ovarian teratomas and androgenetic complete hydatidiform moles (CHM), respectively. Our previous study has reported human parthenogenetic induced pluripotent stem cells from ovarian teratoma-derived fibroblasts and screening of imprinted genes using genome-wide DNA methylation analysis. However, due to the lack of the counterparts of uniparental cells, identification of new imprinted differentially methylated regions has been limited. CHM are inherited from only the paternal genome. In this study, we generated human androgenetic induced pluripotent stem cells (AgHiPSCs) from primary androgenetic fibroblasts derived from CHM. To investigate the pluripotency state of AgHiPSCs, we analyzed their cellular and molecular characteristics. We tested the DNA methylation status of imprinted genes using bisulfite sequencing and demonstrated the androgenetic identity of AgHiPSCs. AgHiPSCs might be an attractive alternative source of human androgenetic embryonic stem cells. Furthermore, AgHiPSCs can be used in regenerative medicine, for analysis of genomic imprinting, to study imprinting-related development, and for disease modeling in humans.
Descripción9 páginas, 5 figuras. Información suplementaria de este artículo accesible en: https://doi.org/10.1038/s41598-020-60363-1
Versión del editorhttp://dx.doi.org/10.1038/s41598-020-60363-1
URIhttp://hdl.handle.net/10261/203818
DOI10.1038/s41598-020-60363-1
E-ISSN2045-2322
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