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Novel insights into the CbrAB expression network in Pseudomonas putida

AuthorsCanosa, Inés ; Barroso, Rocío; Moteagudo-Cascales, Elizabet; García-Mauriño, Sofía M.; Tomás-Gallardo, Laura ; Andújar, Eloísa ; Pérez-Alegre, Mónica ; Santero, Eduardo
Issue Date7-Jul-2019
Citation8th FEMS (2019)
AbstractBackground: The ability to recognize and convert external environmental stimuli into appropriate physiological responses is of fundamental importance for all organisms. In Pseudomonads, the two-component regulatory system CbrAB is responsible to the adaptation to carbon limitation. Global analysis of the regulatory element CbrB in P. putida shows that it controls a collection of ¿N-dependent genes involved in the assimilation of several amino acids but also in a number of other physiological functions, such as chemotaxis or stress tolerance. Conversely, very little is known about the signal that triggers the Cbr response, which is channeled through the histidine kinase CbrA. CbrA represents a new family of sensor HKs as its structure suggests it may link signalling to transport of a molecule. Its N terminus contains a 13 TM domain with similarity to the sodium-proline symporter PutP from E. coli, which is connected to the C terminal catalytic HK domain which could regulate transport through the sodium/solute symporter domain. Objectives: Describe the CbrB regulon for a consensus CbrB-binding sequence description and determine its contribution of in the transcriptional activation. Also , explore the regulation of CbrA expression and the implication of the TM, PAS and HK domains in the signal transduction. Methods: ChIP-seq analysis and point mutagenesis to the DNA sequences necessary for its binding and transcriptional activation. Also, we explore the control of cbrA expression and constructed truncated versions of CbrA to follow the signal transduction. Results: Altogether, we show an extensive view on the molecular mechanism of CbrAB activation.
DescriptionResumen del trabajo presentado en el 8th Congress of European Microbiologists (FEMS), celebrado en Glasgow (Escocia), del 7 al 11 de julio de 2019
Appears in Collections:(CABD) Comunicaciones congresos
(CABIMER) Comunicaciones congresos
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