Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/203129
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Multilocus analysis reveals large genetic diversity in Kluyveromyces marxianus strains isolated from Parmigiano Reggiano and Pecorino di Farindola cheeses |
Autor: | Fasoli, Giuseppe; Barrio, Eladio CSIC ORCID; Tofalo, Rosanna; Suzzi, Giovanna; Belloch, Carmela CSIC ORCID | Palabras clave: | Kluyveromyces marxianus Italian dairy Genetic diversity Inter-RTL mtDNA RFLPs IGS KmSSB1-KmRIO2 sequence Mating type |
Fecha de publicación: | 6-jun-2016 | Editor: | Elsevier | Citación: | International Journal of Food Microbiology 233: 1-10 (2016) | Resumen: | In the present study, we have analysed the genetic diversity in Kluyveromyces marxianus isolated from Parmigiano Reggiano and Pecorino di Farindola cheesemaking environment. Molecular typing methods inter-RTL fingerprint and mtDNA RFLPs, as well as, sequence diversity and heterozygosity in the intergenic region between KmSSB1 and KmRIO2 genes and analysis of the mating locus were applied to 54 K. marxianus strains. Inter-RTL fingerprint revealed a large degree of genetic heterogeneity and clustering allowed differentiation of K. marxianus strains from different geographical origins. In general, inter-LTR profiles were more discriminating than RFLPs of mtDNA; however our results also indicate that both techniques could be complementary unveiling different degrees of genetic diversity. Sequence analysis of the intergenic region between KmSSB1 and KmRIO2 genes revealed 26 variable positions in which a double peak could be observed in the sequence chromatogram. Further analysis revealed the presence of heterozygous strains in the K. marxianus population isolated from Parmigiano Reggiano. On the other hand, all strains isolated from Pecorino di Farindola were homozygous. Two very different groups of haplotypes could be observed as well as mixtures between them. Phylogenetic reconstruction divided K. marxianus dairy strains into two separate populations. A few heterozygous strains in an intermediate position between them could also be observed. Mating type locus analysis revealed a large population of diploid strains containing both MATa and MATα alleles and few haploid strains, most of them presenting the MATα allele. Different scenarios explaining the presence and maintaining of homozygous and heterozygous diploids as well as hybrids between them in the Parmigiano Reggiano K. marxianus population are proposed. A principal component analysis supported the large differences between K. marxianus isolated from Parmigiano Reggiano and Pecorino di Farindola. | URI: | http://hdl.handle.net/10261/203129 | DOI: | 10.1016/j.ijfoodmicro.2016.05.028 | ISSN: | 0168-1605 |
Aparece en las colecciones: | (IATA) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
IJFM2016-Fasoli.pdf | Artículo principal | 983,13 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
17
checked on 11-abr-2024
WEB OF SCIENCETM
Citations
18
checked on 26-feb-2024
Page view(s)
160
checked on 23-abr-2024
Download(s)
290
checked on 23-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Este item está licenciado bajo una Licencia Creative Commons