English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/203079
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Adaptive response and apoptosis in HCT116 cells upon endoplasmic reticulum stress

AuthorsMora-Molina, Rocío; Stöhr, D.; Rhem, M.; López-Rivas, Abelardo
Issue Date15-Oct-2019
CitationXVIII Congreso de la Sociedad Española de Biología Celular (2019)
AbstractObjective: To investigate the interconnection between adaptive and apoptotic responses in tumor cells undergoing ER stress, a situation frequently observed during tumor growth. Material and Methods: the human tumor colorectal HCT116 cell line was used as model system. Knockdown of FLIPL, TRAF2 and RIPK1 were accomplished using small-interference RNA. Cells overexpressing FLIPL were generated by retrovirus infection and selection with puromycin. Multicellular tumor spheroids were prepared by the hanging drop method and grown in 96 well plates coated with agarose until treatment. Results: we have identified FLIPL as an important regulator in the balance between apoptosis and survival in conventional 2D cultures of HCT116 cells. FLIPL downregulation or overexpression provokes sensitization or protection to ER stress, respectively. We have also demonstrated that RIPK1 and TRAF2, two negative modulators of TRAIL-induced apoptosis, have a protective role against ER stress-induced apoptosis. In addition, we show that HCT116 3D spheroids, whose architecture resemble solid tumours, are more resistant to ER stress-induced UPR signalling and apoptosis although more experiments are needed to decipher the molecular and cellular basis of this resistance. Conclusions: (1) FLIPL, TRAF2 and RIPK1 play an adaptive role to delay ER stress-induced apoptosis in HCT116 cells and (2) HCT116-derived spheroids are more resistant to ER stress than standard 2D cultures.
DescriptionTrabajo presentaod en el XVIII Congreso de la Sociedad Española de Biología Celular, celebrado en Badajoz (España), del 15 al 18 de octubre de 2019
URIhttp://hdl.handle.net/10261/203079
Appears in Collections:(CABIMER) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.