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dc.contributor.authorPacheco, Andreiaes_ES
dc.contributor.authorDonzella, Lorenaes_ES
dc.contributor.authorHernández-López, M. Josées_ES
dc.contributor.authorAlmeida, Maria Juditees_ES
dc.contributor.authorPrieto, José Antonioes_ES
dc.contributor.authorRández Gil, Franciscaes_ES
dc.contributor.authorMorrisey, John P.es_ES
dc.contributor.authorSousa, Maria Joaoes_ES
dc.identifier.citationFEMS Yeast Research 20(1): foaa004 (2020)es_ES
dc.descriptionFree-access link to the article: "Hexose transport in Torulaspora delbrueckii: identification of Igt1, a new dual-affinity transporter" Pacheco, Andreia; Donzella, Lorena; Hernández-López, M. José; Almeida, Maria Judite; Prieto, José Antonio; Rández Gil, Francisca; Morrisey, John P.; Sousa, Maria Joao. FEMS Yeast Research 20(1): foaa004 (2020). Available online at: https://doi.org/10.1093/femsyr/foaa004es_ES
dc.description.abstractTorulaspora delbrueckii is a yeast species receiving increasing attention from the biotechnology industry, with particular relevance in the wine, beer and baking sectors. However, little is known about its sugar transporters and sugar transport capacity, frequently a rate-limiting step of sugar metabolism and efficient fermentation. Actually, only one glucose transporter, Lgt1, has been characterized so far. Here we report the identification and characterization of a second glucose transporter gene, IGT1, located in a cluster, upstream of LGT1 and downstream of two other putative hexose transporters. Functional characterization of IGT1 in a Saccharomyces cerevisiae hxt-null strain revealed that it encodes a transporter able to mediate uptake of glucose, fructose and mannose and established that its affinity, as measured by Km, could be modulated by glucose concentration in the medium. In fact, IGT1-transformed S. cerevisiae hxt-null cells, grown in 0.1% glucose displayed biphasic glucose uptake kinetics with an intermediate- (Km = 6.5 ± 2.0 mM) and a high-affinity (Km = 0.10 ± 0.01 mM) component, whereas cells grown in 2% glucose displayed monophasic kinetics with an intermediate-affinity (Km of 11.5 ± 1.5 mM). This work contributes to a better characterization of glucose transport in T. delbrueckii, with relevant implications for its exploitation in the food industry.es_ES
dc.description.sponsorshipAP was supported by PhD fellowship [BD/13 282/2003] from Fundação para a Ciência e para a Tecnologia (FCT), Portugal. LD is supported by the YEASTDOC Marie Curie ITN project which received funding from the European Union's Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie grant agreement no 764 927. This work was supported by the strategic programme UID/BIA/0 4050/2013 (POCI-01-0145-FEDER-0 07569) and project PTDC/BIA-MIC/32 059/2017 funded by national funds through the FCT,I.P. and by the ERDF through the COMPETE2020–Programa Operacional Competitividade e Internacionalização (POCI) and Sistema de Apoio à Investigação Científica e Tecnológica (SAICT).es_ES
dc.publisherOxford University Presses_ES
dc.subjectTorulaspora delbrueckiies_ES
dc.subjectGlucose transportes_ES
dc.subjectHexose transporteres_ES
dc.subjectTransport kineticses_ES
dc.subjectDual affinityes_ES
dc.subjectGlucose transporter gene clusteres_ES
dc.titleHexose transport in Torulaspora delbrueckii: identification of Igt1, a new dual-affinity transporteres_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderFundação para a Ciência e a Tecnologia (Portugal)es_ES
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