English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/202702
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Subretinal Transplant of Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium on Nanostructured Fibrin-Agarose

AuthorsGarcía-Delgado, Ana B.; Cerda, Berta de la ; Alba Amador, Julia; Valdés-Sánchez, María Lourdes; Fernández-Muñoz, Beatriz ; Relimpio, Isabel; Rodríguez de la Rúa, Enrique; Díez-Lloret, Andrea ; Calado, Sofia M.; Sánchez-Pernaute, Rosario; Bhattacharya, Shom Shanker ; Díaz-Corrales, Francisco J.
KeywordsMacular degeneration
Retinal pigment epithelium
Induced pluripotent stem cells
Subretinal implant
Fibrin-agarose hydrogel
Issue Date10-May-2019
PublisherMary Ann Liebert
CitationTissue Engineering - Part A 25(9-10): 799-808 (2019)
AbstractDamage to the retinal pigment epithelium (RPE) in age-related macular degeneration and other diseases results in photoreceptor cell death and blindness. Replacement of RPE is therefore being explored as a therapy for several retinal diseases. To move toward a future personalized autologous transplant approach, we have prepared a biocompatible implant using RPE derived from induced pluripotent stem cells (iPSCs) reprogrammed from a healthy donor's monocytes. The correct positioning of the polarized RPE is essential to fulfill its role and protect photoreceptors from degeneration. Hence, we have used a biocompatible hydrogel matrix of fibrin and agarose that allows the surgical placement of an RPE sheet in the subretinal space, keeping its functional orientation. Our aim was to demonstrate safety and viability of the transplant in preclinical models. Pigs were used to test the feasibility of a regular vitreoretinal surgery. Our results show that this implant is suitable for subretinal transplantation allowing human RPE cells to survive and maintain their phenotype and orientation without any local or systemic adverse events. The ability to transplant the iPSC-derived RPE sheet in its natural orientation will surely increase the chance to obtain a therapeutic effect in future translational studies. In the promising field of cellular therapy for retinal degenerative diseases, a new biomaterial is proposed as a scaffold to grow and surgically introduce a monolayer of retinal pigment epithelial cells into the subretinal space, keeping the orientation of the cells for a proper functional integration of the transplant. The use of induced pluripotent stem cells as the starting material for retinal pigment epithelial cells is intended to advance toward a personalized medicine approach.
Publisher version (URL)http://dx.doi.org/10.1089/ten.tea.2019.0007
Identifiersdoi: 10.1089/ten.tea.2019.0007
e-issn: 1937-335X
issn: 1937-3368
Appears in Collections:(CABIMER) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.