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Title

Gold nanoparticles for the in situ polymerization of near-infrared responsive hydrogels based on fibrin

AuthorsEscudero-Duch, Clara; Martín-Saavedra, Francisco; Prieto, Martin; Sánchez-Casanova, Silvia; López, Daniel ; Sebastian, Victor; Arruebo, Manuel; Santamaría, Jesús; Vilaboa, Nuria
KeywordsNear infrared
Hollow gold nanoparticles
Thrombin
Fibrin
Issue Date2019
PublisherElsevier
CitationActa Biomaterialia 100: 306-315 (2019)
AbstractNon-invasiveness and relative safety of photothermal therapy, which enables local hyperthermia of target tissues using a near infrared (NIR) laser, has attracted increasing interest. Due to their biocompatibility, amenability of synthesis and functionalization, gold nanoparticles have been investigated as therapeutic photothermal agents. In this work, hollow gold nanoparticles (HGNP) were coated with poly-L-lysine through the use of COOH-Poly(ethylene glycol)-SH as a covalent linker. The functionalized HGNP, which peak their surface plasmon resonance at 800 nm, can bind thrombin. Thrombin-conjugated HGNP conduct in situ fibrin polymerization, facilitating the process of generating photothermal matrices. Interestingly, the metallic core of thrombin-loaded HGNP fragmentates at physiological temperature. During polymerization process, matrices prepared with thrombin-loaded HGNP were loaded with genetically-modified stem cells that harbour a heat-activated and ligand-dependent gene switch for regulating transgene expression. NIR laser irradiation of resulting cell constructs in the presence of ligand successfully triggered transgene expression in vitro and in vivo.
Publisher version (URL)http://dx.doi.org/10.1016/j.actbio.2019.09.040
URIhttp://hdl.handle.net/10261/202139
Identifiersdoi: 10.1016/j.actbio.2019.09.040
e-issn: 1878-7568
issn: 1742-7061
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(ICMA) Artículos
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