Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/201725
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | CD44-high neural crest stem-like cells are associated with tumour aggressiveness and poor survival in neuroblastoma tumours |
Autor: | Vega, Francisco M. CSIC ORCID; Colmenero-Repiso, Ana CSIC; Gómez-Muñoz, María A. CSIC ORCID; Rodríguez-Prieto, Ismael CSIC; Aguilar Morante, Diana CSIC ORCID; Ramírez, Gema; Márquez-Vega, Catalina; Cabello, Rosa; Pardal Redondo, Ricardo CSIC ORCID | Palabras clave: | CD44 Neuroblastoma Differentiation Neural crest stem cells Biomarkers Intra-tumour heterogeneity Cancer |
Fecha de publicación: | nov-2019 | Editor: | Elsevier | Citación: | EBioMedicine 49: 82-95 (2019) | Resumen: | [Background] Neuroblastoma is a paediatric tumour originated from sympathoadrenal precursors and characterized by its heterogeneity and poor outcome in advanced stages. Intra-tumoral cellular heterogeneity has emerged as an important feature in neuroblastoma, with a potential major impact on tumour aggressiveness and response to therapy. CD44 is an adhesion protein involved in tumour progression, metastasis and stemness in different cancers; however, there has been controversies about the significance of CD44 expression in neuroblastoma and its relationship with tumour progression. [Methods] We have performed transcriptomic analysis on patient tumour samples studying the outcome of patients with high CD44 expression. Adhesion, invasion and proliferation assays were performed in sorted CD44high neuroblastoma cells. Tumoursphere cultures have been used to enrich in undifferentiated stem-like cells and to asses self-renewal and differentiation potential. We have finally performed in vivo tumorigenic assays on cell line-derived or Patient-derived xenografts. [Findings] We show that high CD44 expression is associated with low survival in high-grade human neuroblastoma, independently of MYCN amplification. CD44 is expressed in a cell population with neural crest stem-like features, and with the capacity to generate multipotent, undifferentiated tumourspheres in culture. These cells are more invasive and proliferative in vitro. CD44 positive cells obtained from tumours are more tumorigenic and metastatic, giving rise to aggressive neuroblastic tumours at high frequency upon transplantation. [Interpretation] We describe an unexpected intra-tumoural heterogeneity within cellular entities expressing CD44 in neuroblastoma, and propose that CD44 has a role in neural crest stem-like undifferentiated cells, which can contribute to tumorigenesis and malignancy in this type of cancer. |
Versión del editor: | https://doi.org/10.1016/j.ebiom.2019.10.041 | URI: | http://hdl.handle.net/10261/201725 | DOI: | 10.1016/j.ebiom.2019.10.041 | E-ISSN: | 2352-3964 |
Aparece en las colecciones: | (IBIS) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
1-s2.0-S235239641930711X-main.pdf | 4,85 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
24
checked on 15-abr-2024
SCOPUSTM
Citations
30
checked on 13-abr-2024
WEB OF SCIENCETM
Citations
28
checked on 27-feb-2024
Page view(s)
148
checked on 19-abr-2024
Download(s)
112
checked on 19-abr-2024