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dc.contributor.authorFaria, Melissa S.es_ES
dc.contributor.authorPrats, Evaes_ES
dc.contributor.authorNovoa-Luna, Karen Adrianaes_ES
dc.contributor.authorBedrossiantz, Juliettees_ES
dc.contributor.authorGómez-Canela, Cristianes_ES
dc.contributor.authorGómez-Olivan, L. M.es_ES
dc.contributor.authorRaldúa, Demetrioes_ES
dc.identifier.citationScience of the Total Environment 650: 87-96 (2019)es_ES
dc.description.abstractThe present paper describes the vibrational startle response assay (VSRA), a new robust, simple and automated in vivo medium- to high-throughput procedure for assessment of the escape response and its habituation in zebrafish larvae. Such behaviors enable fish larvae to escape from predator strikes in aquatic ecosystems. The assay is based on measuring the distance moved by each larva during the startle response evoked by repetitive vibrational stimuli. The iterative reduction observed in the response to a series of tapping stimulus in VSRA met the main criteria of habituation. Subsequently, the analysis of concordance using a battery of neuroactive compounds modulating different neurotransmitter systems demonstrated that the results of VSRA are highly predictive of the effects on other vertebrates. Finally, as a proof of concept, VSRA was used to test two relevant environmental pollutants at different concentrations. The results demonstrated that VSRA is suitable for concentration-response analysis of environmental pollutants, opening the possibility to determine the potency and the associated hazard of impaired escape response for the different compounds. Therefore, we suggest that VSRA could be a valuable tool for screening of chemical compounds capable of compromising predator avoidance behavior. © 2018 Elsevier B.V.es_ES
dc.description.sponsorshipThis work was supported by the Spanish Government ( CTM2017-83242-R ) and by the NATO SfP project MD.SFPP 984777 . M.F acknowledges financial support from the Beatriu de Pinós programme (grant No: 2016 BP 00233 ) provided by the Secretariat of Universities and Research department of the Ministry for Business and Knowledge, Catalonia Government. K.A.N.L was supported by the grant 291212 from the Mixed Fund programme for mobility (CONACYT-2017). J.B acknowledges financial support from Bouygues Foundation 's bursary, in the frame of the Erasmus Traineeship ( FTOULOUS03 ).es_ES
dc.subjectEscape responsees_ES
dc.titleDevelopment of a vibrational startle response assay for screening environmental pollutants and drugs impairing predator avoidancees_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidFaria, Melissa S. [0000-0003-1451-4427]es_ES
dc.contributor.orcidPrats, Eva [0000-0001-7838-2027]es_ES
dc.contributor.orcidRaldúa, Demetrio [0000-0001-5256-1641]es_ES
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