English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/201188
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Antibiotic resistance gene distribution in agricultural fields and crops. A soil-to-food analysis

AuthorsCerqueira, Francisco; Matamoros, Víctor CSIC ORCID ; Bayona Termens, Josep María; Berendonk, Thomas Ulrich; Elsinga, Goffe; Hornstra, Luc M.; Piña, Benjamín CSIC ORCID
KeywordsSoil
Antibiotic resistance genes
Microbiomes
Endophytes
Irrigation water
qPCR
Rhizosphere
Issue DateOct-2019
PublisherElsevier
CitationEnvironmental Research 177: 108608 (2019)
AbstractDespite the social concern about the generalization of antibiotic resistance hotspots worldwide, very little is known about the contribution of different potential sources to the global risk. Here we present a quantitative analysis of the distribution of Antibiotic Resistance Genes (ARGs) in soil, rhizospheric soil, roots, leaves and beans in tomato, lettuce and broad beans crops (165 samples in total), grown in nine commercial plots distributed in four geographical zones in the vicinity of Barcelona (North East Spain). We also analyzed five soil samples from a nearby forest, with no record of agricultural activities. DNA samples were analyzed for their content in the ARGs sul1, tetM, qnrS1, blaCTX-M-32, blaOXA-58, mecA, and blaTEM, plus the integron intI1, using qPCR methods. In addition, soil microbiomes from the different plots were analyzed by amplicon-targeted 16S rRNA gene sequencing. Our data show a decreasing gradient of ARG loads from soil to fruits and beans, the latter showing only from 0.1 to 0.01% of the abundance values in soil. The type of crop was the main determinant for both ARG distribution and microbiome composition among the different plots, with minor contributions of geographic location and irrigation water source. We propose that soil amendment and/or fertilization, more than irrigation water, are the main drivers of ARG loads on the edible parts of the crop, and that they should therefore be specifically controlled. © 2019 Elsevier Inc.
Publisher version (URL)https://doi.org/10.1016/j.envres.2019.108608
URIhttp://hdl.handle.net/10261/201188
DOIhttp://dx.doi.org/10.1016/j.envres.2019.108608
Appears in Collections:(IDAEA) Artículos
Files in This Item:
File Description SizeFormat 
Antibiotic Resistance Gene distribution in agricultural fields and 1 crops. A soil-to-food analysis.pdf Embargoed until October 1, 2021Artículo principal2,17 MBAdobe PDFThumbnail
View/Open    Request a copy
1-s2.0-S0013935119304050-mmc1.docxMaterial suplementario1,26 MBMicrosoft Word XMLView/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.