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Study of self-compatibility in almond progenies from self-fertilization by fluorescence microscopy and stylar ribonuclease assay

AuthorsOrtega, Encarnación ; Martínez-Gómez, Pedro ; Dicenta, Federico ; Bošković, Radovan I.; Tobutt, Kenneth R.
Prunus dulcis
Pollen tube growth
Stylar ribonucleases
Issue Date2002
PublisherInternational Society for Horticultural Science
CitationActa Horticulturae 591: 229-232 (2002)
AbstractSelf-incompatibility in almond is gametophytic and controlled by a single S-locus with several codominant alleles encoding for specific ribonucleases and a dominant self-fertility allele (Sf) that does not encode for ribonuclease activity. In most studies of inheritance of self-compatibility, crosses between self-compatible and self-incompatible cultivars were studied. However, progenies from self-pollination are scarce. In this work, self-compatibility of 214 seedlings from self-pollination of 30 self-compatible selections, obtained by the CEBAS Almond Breeding Programme, was studied by means of fluorescence microscopy and stylar ribonuclease assay. Results showed that self-fertilization of self-compatible genotypes guarantees self-compatibility in the offspring and allows homozygous individuals for the Sf allele to be obtained. Both methods are reliable for assessing self-compatibility in this species. Furthermore, stylar ribonuclease assay allowed the determination of the S genotype in the progeny and detected some unexpected S-genotypes.
Description4 pages.-- En: ISHS Acta Horticulturae 591: III International Symposium on Pistachios and Almonds.
Publisher version (URL)http://www.actahort.org/books/591/591_32.htm
Appears in Collections:(CEBAS) Artículos
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