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Title

Low temperature storage of almond pollen

AuthorsMartínez-Gómez, Pedro ; Gradziel, Thomas M.
KeywordsPrunus dulcis
Pollen
Viability
Pollen germination
Cryopreservation
Breeding
Issue DateJul-2002
PublisherAmerican Society for Horticultural Science
CitationHortScience 37(4): 691-692 (2002)
AbstractPollen of the California almond cultivars Nonpareil, Ne Plus Ultra, Sonora, and Peerless was evaluated for viability following storage over 12 months at 4, 0, -20, and -80 °C. The proportion of viable pollen exceeded 80% for all cultivars and for all temperatures evaluated after 2 months of storage. Following 12 months of storage at 4 °C, germination decreased to 8% for `Nonpareil', 10% for `Ne Plus Ultra', 50% for `Sonora', with no germination observed for `Peerless'. Storage at sub-freezing temperatures maintained pollen viability above 70% in `Nonpareil', `Ne Plus Ultra', and `Sonora' and above 40% in `Peerless'. Cultivars differed significantly in their tolerance to low temperature pollen storage. Within cultivars, differences in pollen germination following storage at 0, -20, or -80 °C were nonsignificant.
Description2 pages.
Publisher version (URL)http://hortsci.ashspublications.org/cgi/content/abstract/37/4/691
URIhttp://hdl.handle.net/10261/20076
ISSN0018-5345
Appears in Collections:(CEBAS) Artículos
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