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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/20054
Título

The highly virulent variola and monkeypox viruses express secreted inhibitors of type I interferon

AutorFernández de Marco, M. del Mar; Alejo, Alí; Hudson, Paul N.; Damon, Inger K.; Alcamí, Antonio
Palabras claveInterferon
Variola
Fecha de publicación17-dic-2009
EditorFederation of American Societies for Experimental Biology
CitaciónThe FASEB journal fj.09-144733
ResumenVariola virus (VARV) caused smallpox, one of the most devastating human diseases and the first to be eradicated, but its deliberate release represents a dangerous threat. Virulent orthopoxviruses infecting humans, such as monkeypox virus (MPXV), could fill the niche left by smallpox eradication and the cessation of vaccination. However, immunomodulatory activities and virulence determinants of VARV and MPXV remain largely unexplored. We report the molecular characterization of the VARV- and MPXV-secreted type I interferon-binding proteins, which interact with the cell surface after secretion and prevent type I interferon responses. The proteins expressed in the baculovirus system have been purified, and their interferon-binding properties characterized by surface plasmon resonance. The ability of these proteins to inhibit a broad range of interferons was investigated to identify potential adaptation to the human immune system. Furthermore, we demonstrate by Western blot and activity assays the expression of the type I interferon inhibitor during VARV and MPXV infections. These findings are relevant for the design of new vaccines and therapeutics to smallpox and emergent virulent orthopoxviruses because the type I interferon-binding protein is a major virulence factor in animal models, vaccination with this protein induces protective immunity, and its neutralization prevents disease progression.—Fernández de Marco, M. M., Alejo, A., Hudson, P., Damon, I. K., Alcami, A. The highly virulent variola and monkeypox viruses express secreted inhibitors of type I interferon.
Versión del editorhttp://dx.doi.org/10.1096/fj.09-144733
URIhttp://hdl.handle.net/10261/20054
DOI10.1096/fj.09-144733
ISSN1530-6860
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