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Rooting ability of some Persian walnut cultivars and peroxidase analysis

AutorVahdati, K.; Zamani, Z.; Khalid, Amari; McGranahan, G.; Martínez-Gómez, Pedro
Palabras claveRooting
Walnut cultivars
biochemical marker
Fecha de publicación2002
CitaciónPajouhesh-va-Sazandegi 15(54): 32-36 (2002)
ResumenThe rooting ability of three commercial, cultivars of Juglans regia L. ('Sunland', Chandler and Vina) was studied in vitro Microshoots were cultured on an induction medium (MS gelled with Kobe Agar and supplemented with 3 mg 1-1 IBA) for a week and rooting was evaluated after four weeks. Walnut cultivars exhibited significant differences in rooting, so that Sunland rooted most easily (90%), followed by Chandler (64%) and Vina (21%). Peroxidase enzyme has been introduced as a biochemical marker for rooting isozymes of this enzyme was analyzed in a horizontal starch gel. The presence of the peroxidase bands and polymorphic behavior was different in the easy - to - root Sunland cultivar. On the other hand, the amount of peroxidase enzyme activity was monitored during the induction stage of rooting in studied cultivars. Peroxidase activity was higher in the easiest -to - root cultivar over the rooting stages. The implication of these results in the micropropagation of walnut was discussed.
Descripción5 pages.-- In: Agronomy and Horticulture.
Versión del editorhttp://www.runetwork.de/html/en/index.html?journal_id=240&page=herausgeber
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