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dc.contributor.authorMartínez-Gómez, Pedro-
dc.contributor.authorRubio, Manuel-
dc.contributor.authorDicenta, Federico-
dc.contributor.authorAparicio Herrero, Frederic-
dc.contributor.authorPallás Benet, Vicente-
dc.date.accessioned2010-01-14T09:50:45Z-
dc.date.available2010-01-14T09:50:45Z-
dc.date.issued2003-
dc.identifier.citationActa Horticulturae 622: 397-401 (2003)en_US
dc.identifier.issn0567-7572-
dc.identifier.urihttp://hdl.handle.net/10261/20039-
dc.description5 pages.-- En: ISHS Acta Horticulturae 622: XXVI International Horticultural Congress: Genetics and Breeding of Tree Fruits and Nutsen_US
dc.description.abstractPlum pox potyvirus (PPV), is the causal agent of the Sharka disease, which is one of the most destructive viral disease of Prunus stone fruits, mainly apricot, in Europe. No control means have already been developed except for the complete destruction of infected trees and the application of strict quarantine control measures. On the other hand, the develop and growth of new resistance cultivars could be the definitive solution to the problem. In this moment search for PPV resistance is one of the most important objectives in all the European apricot breeding programs. The routine application of an adequate detection method during the evaluation of PPV resistance is a critical step in these breeding programs. During last years, a large number of serological and PCR-based methods have been described for their application in these programs, whereas methods based on the molecular hybridization techniques have been less considered despite that have recently shown to be a very good alternative for routine diagnosis. In this work, the comparison between non-radioactive molecular hybridization, ELISA-DASI with monoclonal antibodies, and IC-PCR, was performed in terms of sensitivity, reliability, cost, and routine application. Results showed the higher sensitivity of the IC-PCR although was more time consuming and expensive. Molecular hybridization and ELISA showed similar sensitivity, reliability and routine application, but the cost of the molecular hybridization was lower than ELISA-DASI.en_US
dc.format.extent259768 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherInternational Society for Horticultural Scienceen_US
dc.rightsclosedAccessen_US
dc.subjectSharkaen_US
dc.subjectPrunus armeniacaen_US
dc.subjectELISA-DASIen_US
dc.subjectMolecular hybridizationen_US
dc.subjectRT-PCRen_US
dc.subjectSelectionen_US
dc.titleComparative analysis of three diagnostic methods for the evaluation of Plum pox virus (PPV) resistance in apricot breeding programsen_US
dc.typeartículoen_US
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://www.actahort.org/books/622/622_35.htmen_US
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
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