English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/20037
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Identification of self-incompatibility alleles in almond and related Prunus species using PCR

AutorMartínez-Gómez, Pedro ; Dandekar, A. M.; Gradziel, Thomas M.; López Valero, Manuela; Batlle, I.; Alonso, J. M.; Ortega, Encarnación ; Sánchez-Pérez, Raquel ; Dicenta, Federico ; Socías i Company, R.
Palabras claveMolecular markers
Breeding program
Assisted selection
Prunus dulcis
Fecha de publicación2003
EditorInternational Society for Horticultural Science
CitaciónActa Horticulturae 622: 353-357 (2003)
ResumenSelf-incompatibility in almond is of the gametophytic type and acts to prevent self-fertilization. This trait is controlled by a single locus with multiple codominant alleles and is expressed within the styles as S-RNases. These glycoproteins are responsible for the inactivation of self-pollen tube growth. Self-incompatibility alleles (S-alleles) can be identified through controlled crosses with a series of known S-genotypes. Molecular methods have recently been developed allowing both stylar S-RNases electrophoresis and S-allele specific amplification using PCR. In this study, the identification of S-genotypes of almond cultivars and related Prunus species was achieved using the specific PCR primers AS1II and AmyC5R. Plant material analyzed included 18 almond cultivars from different geographical origins and 12 Prunus species. Results identify 8 unique S-alleles in almond and 6 in related Prunus species. Findings also clarify the genetic relationships among almond and related Prunus species. The nucleotide sequences of these primers appear to be highly conserved in almond and its close relatives. The applications of these PCR markers in the UC-Davis almond-breeding program are discussed.
Descripción5 pages.-- En: ISHS Acta Horticulturae 622: XXVI International Horticultural Congress: Genetics and Breeding of Tree Fruits and Nuts.
Versión del editorhttp://www.actahort.org/books/622/622_41.htm
URIhttp://hdl.handle.net/10261/20037
ISSN0567-7572
Aparece en las colecciones: (CEBAS) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.