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Título: | Different oxygen concentrations in lupin nodules functioning: genetic and physiological aspects |
Autor: | Guasch, Luis; Fernández-Pascual, Mercedes CSIC ; Lucas, M. Mercedes CSIC ORCID; Hernández-Jiménez, M. J.; Felipe, Mª Rosario de | Fecha de publicación: | 1997 | Editor: | Kluwer Academic Publishers | Citación: | Biological Nitrogen Fixation for the 21st Century: 500-500 (1997) | Resumen: | The effects of O2 concentrations (1, 21, 40% applied for 1, 3 and 10d) on nitrogenase activity (N2ase). O2 diffusion resistance (ODR), glycoprotein content, enod2 expression and nodule structure of Lupinus albus L plants have been studied. N2ase and ODR were measured using a flow-through system installed in a greenhouse. When 1% O2 was applied for 1d, N2ase was completely inhibited. However as O2 concentration was increased from 1% to 21%, nitrogenase activity was totally recovered. The recovery was only 30% after 3d of 1% O2 application and it was completely lost after 10d. When 40% O2 was applied during 1 and 3d a decrease in N2ase was detected, but after 10d N2ase was similar to the control. ODR increased highly with 3d of 40% O2 and it was double than control after 10d. Glycoprotein content decreased along 1% O2 treatment. In 40% O2 treated plants an increase was observed after 3d but after 10d there were no significant differences to the control. Expression of enod2 also diminished with 1% O2 and its evolution with 40% O2 followed a similar pattern to glycoprotein. A clear correlation between nodule functioning and structure was observed using different microscopies: At LM with 1% O2 nodule cortex changed progressively into an aerenchyma tissue. At 3d, middle cortex cells in the 40% O2 treatment presented an increase in size and their intercellular spaces (IS) were more occluded by glycoprotein (data confirmed by western-blot). Nodule structure was more similar to control after 10d of 40% O2. At TEM, 1% O2 bacteroids started to degrade after 3d, and after 10d bacteroids degradation was general. After 10d of 40% O2 bacteroids were larger (15%) and contained numerous polyhydroxybutirate grains. At Cryo-SEM, 1% O2 infected cells appeared highly vacuolated. The sizes of the IS of infected cells in 40% and 21%O2 were similar, although the volume of infected cells was bigger. So the IS volume/cell volume ratio diminished reducing O2 diffusion into the infected cells. | Descripción: | Trabajo presentado en el 11th International Congress on Nitrogen Fixation, celebrado en Paris (Francia), del 20 al 25 de julio de 1997 | Versión del editor: | https://doi.org/10.1007/978-94-011-5159-7_310 | URI: | http://hdl.handle.net/10261/200208 | DOI: | 10.1007/978-94-011-5159-7_310 | Identificadores: | doi: 10.1007/978-94-011-5159-7_310 isbn: 0-7923-4834-6 |
Aparece en las colecciones: | (ICA) Libros y partes de libros |
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