Integration of Cryo-EM Model Building Software in Scipion.

Abstract

Advances in cryo-electron microscopy (cryoEM have made possible to obtain structures of large biological macromolecules at near-atomic resolution. This "resolution-revolution'' has encouraged the use and development of modeling tools able to produce high-quality atomic models from cryoEM density maps. Unfortunately, many practical problems appear when combining different packages in the same processing workflow, which make difficult the use of these tools by non-experts and, therefore, reduce their utility. We present here a major extension of the image processing framework Scipion that provides inter-package integration in the model building area and full tracking of the complete workflow, from image processing to structure validation.