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dc.contributor.authorBrenes-Álvarez, Manueles_ES
dc.contributor.authorVioque, Agustínes_ES
dc.contributor.authorMuro-Pastor, Alicia M.es_ES
dc.identifier.citationMolecular Biology and Physiology, 11:e02599-19.(2020)es_ES
dc.description.abstractYfr1 is a strictly conserved small RNA in cyanobacteria. A bioinformatic prediction to identify possible interactions of Yfr1 with mRNAs was carried out by using the sequences of Yfr1 from several heterocyst-forming strains, including Nostoc sp. strain PCC 7120. The results of the prediction were enriched in genes encoding outer membrane proteins and enzymes related to peptidoglycan biosynthesis and turnover. Heterologous expression assays with Escherichia coli demonstrated direct interactions of Yfr1 with mRNAs of 11 of the candidate genes. The expression of 10 of them (alr2458, alr4550, murC, all4829, all2158, mraY, alr2269, alr0834, conR, patN) was repressed by interaction with Yfr1, whereas the expression of amiC2, encoding an amidase, was increased. The interactions between Yfr1 and the 11 mRNAs were confirmed by site-directed mutagenesis of Yfr1. Furthermore, a Nostoc strain with reduced levels of Yfr1 had larger amounts of mraY and murC mRNAs, supporting a role for Yfr1 in the regulation of those genes. Nostoc strains with either reduced or increased expression of Yfr1 showed anomalies in cell wall completion and were more sensitive to vancomycin than the wild-type strain. Furthermore, growth in the absence of combined nitrogen, which involves the differentiation of heterocysts, was compromised in the strain overexpressing Yfr1, and filaments were broken at the connections between vegetative cells and heterocysts. These results indicate that Yfr1 is an important regulator of cell wall homeostasis and correct cell wall remodeling during heterocyst differentiationes_ES
dc.publisherAmerican Society for Microbiologyes_ES
dc.relation: MINECO/ICTI2013-2016/BFU2013-48282-C2-1es_ES
dc.relation.isversionofPublisher's versiones_ES
dc.subjectHeterocyst differentiationes_ES
dc.subjectRegulatory RNAses_ES
dc.subjectSmall RNAses_ES
dc.titleThe Integrity of the Cell Wall and Its Remodeling during Heterocyst Differentiation Are Regulated by Phylogenetically Conserved Small RNA Yfr1 in Nostoc sp. Strain PCC 7120es_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidBrenes-Álvarez, Manuel [0000-0002-6452-5557]es_ES
dc.contributor.orcidVioque, Agustín [0000-0002-3975-7348]es_ES
dc.contributor.orcidMuro-Pastor, Alicia M. [0000-0003-2503-6336]es_ES
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