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Title

Detoxification of 1,1,2-trichloroethane to ethene in a bioreactor co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains

AuthorsMortan, Siti Hatijah; Martín-González, Lucía; Vicent, T.; Caminal, Glòria CSIC ORCID; Nijenhuis, Ivonne; Adrian, Lorenz; Marco-Urrea, Ernest
Keywords1,1,2-Trichloroethane
Dehalococcoides
Dehalogenimonas
Fed-batch reactor
Organohalide-respiring bacteria
Issue Date5-Jun-2017
PublisherElsevier
CitationJournal of Hazardous Materials 331: 218-225 (2017)
Abstract1,1,2-Trichloroethane (1,1,2-TCA) is a non-flammable organic solvent and common environmental contaminant in groundwater. Organohalide-respiring bacteria are key microorganisms to remediate 1,1,2-TCA because they can gain metabolic energy during its dechlorination under anaerobic conditions. However, all current isolates produce hazardous end products such as vinyl chloride, monochloroethane or 1,2-dichloroethane that accumulate in the medium. Here, we constructed a syntrophic co-culture of Dehalogenimonas and Dehalococcoides mccartyi strains to achieve complete detoxification of 1,1,2-TCA to ethene. In this co-culture, Dehalogenimonas transformed 1,1,2-TCA via dihaloelimination to vinyl chloride, whereas Dehalococcoides reduced vinyl chloride via hydrogenolysis to ethene. Molasses, pyruvate, and lactate supported full dechlorination of 1,1,2-TCA in serum bottle co-cultures. Scale up of the cultivation to a 5-L bioreactor operating for 76 d in fed-batch mode was successful with pyruvate as substrate. This synthetic combination of bacteria with known complementary metabolic capabilities demonstrates the potential environmental relevance of microbial cooperation to detoxify 1,1,2-TCA. © 2017 Elsevier B.V.
Publisher version (URL)https://doi.org/10.1016/j.jhazmat.2017.02.043
URIhttp://hdl.handle.net/10261/199117
DOIhttp://dx.doi.org/10.1016/j.jhazmat.2017.02.043
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