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Measuring local-directional resolution and local anisotropy in cryo-EM maps

AuthorsS. Sorzano, Carlos Oscar; Vilas, José Luis; Tagare, Hemant D.; Vargas, Javier; Carazo, José María
Issue DateJan-2020
PublisherNature Publishing Group
CitationNature communications 11 (1): 55 (2020)
AbstractThe introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation.
Publisher version (URL)http://dx.doi.org/10.1038/s41467-019-13742-w
Appears in Collections:(CNB) Artículos
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