English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/197513
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi

AuthorsFisher, Matthew C.; Ghosh, Pria; Shelton, Jennifer M. G.; Bates, Kieran A.; Brookes, Lola; Wierzbicki, Claudia; Rosa, Gonçalo M.; Farrer, Rhys A.; Aanensen, David M.; Alvarado-Rybak, Mario; Bataille, Arnaud; Berger, Lee R.; Böll, Susanne; Bosch, Jaime ; Clare, Frances C.; Courtois, Elodie A.; Crottini, Angelica; Cunningham, Andrew A.; Doherty-Bone, Thomas M.; Gebresenbet, Fikirte; Gower, David J.; Höglund, Jacob; James, Timothy Y.; Jenkinson, Thomas S.; Kosch, Tiffany A.; Lambertini, Carolina; Laurila, Anssi; Lin, Chun-Fu; Loyau, A.; Martel, An; Meurling, Sara; Miaud, Claude; Minting, Pete; Ndriantsoa, Serge; O’Hanlon, Simon; Pasmans, Frank; Rakotonanahary, Tsanta; Rabemananjara, Falitiana C. E.; Ribeiro, Luisa P.; Schmeller, Dirk S.; Schmidt, Benedikt R.; Skerratt, Lee; Smith, Freya; Soto-Azat, Claudio; Tessa, Giulia; Toledo, Luis Felipe; Valenzuela-Sánchez, Andrés; Verster, Ruhan; Vörös, Judit; Waldman, Bruce; Webb, Rebecca J.; Weldon, Che; Wombwell, Emma; Zamudio, Kelly R.; Longcore, Joyce E.; Garner, Trenton W. J.
KeywordsMicrobiology
Ecological epidemiology
Biological techniques
Issue Date2018
PublisherSpringer Nature
CitationScientific Reports 8: 7772 (2018)
AbstractParasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens.
Description© The Author(s) 2018.
Publisher version (URL)https://doi.org/10.1038/s41598-018-24472-2
URIhttp://hdl.handle.net/10261/197513
DOI10.1038/s41598-018-24472-2
E-ISSN2045-2322
Appears in Collections:(MNCN) Artículos
Files in This Item:
File Description SizeFormat 
Development_Fisher_Art2018.pdf1,48 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.