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Title

Detecting transient protein–protein interactions by X-ray absorption spectroscopy: The cytochrome c6-photosystem I complex

AuthorsDíaz-Moreno, Irene CSIC ORCID; Díaz-Quintana, Antonio; Subías, G. CSIC ORCID; Mairs, Trevor; Rosa, Miguel A. de la CSIC ORCID; Díaz-Moreno, Sofía
KeywordsCytochrome c6
Photosystem I
Transient interactions
X-ray absorption spectroscopy
Issue DateMay-2006
PublisherElsevier
CitationFEBS Letters 580(13): 3023-3028 (2006)
AbstractReliable analysis of the functionality of metalloproteins demands a highly accurate description of both the redox state and geometry of the metal centre, not only in the isolated metalloprotein but also in the transient complex with its target. Here, we demonstrate that the transient interaction between soluble cytochrome c6 and membrane-embedded photosystem I involves subtle changes in the heme iron, as inferred by X-ray absorption spectroscopy (XAS). A slight shift to lower energies of the absorption edge of Fe2+ in cytochrome c6 is observed upon interaction with photosystem I. This work constitutes a novel application of XAS to the analysis of weak complexes in solution.
Description6 pages, 1 table, 3 fiures, 1 appendix.-- Edited by Peter Brzezinski.
Publisher version (URL)http://dx.doi.org/10.1016/j.febslet.2006.04.045
URIhttp://hdl.handle.net/10261/19744
DOI10.1016/j.febslet.2006.04.045
ISSN0014-5793
Appears in Collections:(ICMA) Artículos
(IBVF) Artículos

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