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Title

One Plate-double Nutrient Endospore Activation Method

AuthorsRekadwad, Bhagwan N.; González Grau, Juan Miguel ; Khobragade, Chandrahasya N.
KeywordsEndospore
Thermophiles
Extremophiles
Survival under low temperature
Hot springs
Issue Date5-Jan-2020
PublisherBio-protocol LLC
CitationBio-101: e3474 (2020)
AbstractThis paper presents a simple, swift, improved and inexpensive one plate-double nutrient endospore activation method (ODNEAM) for activation of thermophilic endospore (thermospore). Revival and cultivation of dormant bacteria experiencing un-culturable state is a difficult task. Long-term preservation at low temperature (4 °C or below) under laboratory conditions pushes most of the gram-positive bacteria to form endospores and enter dormancy. After experienced dormancy and crossed specific time (more than a month) it’s difficult for those thermospores to resurrect into a living cell. The effect of low temperature, a double quantity of nutrients and high humidity (80 %) was re-revaluated to foster germination of thermospores. Employed parameters successfully revived thermospores which experience dormancy or unable to initiate the germination process upon onset of favorable conditions. This method has crucial importance for activation of the dormant thermospore. Long-term preservation under low temperature or no sub-culturing at a fixed time interval of thermophilic bacteria may be a cause for least revival rate and the progressively diminished revival rate. The inactivation of cells after long-term preservation and probable loss to science would be avoided using this one plate-double nutrient endospore activation method. This is a tribute to Harold F. Foerster−A renowned microbiologist (1983).
Description10 páginas.- 3 figuras.- 1 tabla.- 8 referencias.- El artículo está disponible online en la página del editor se puede descargar gratuitamente en pdf. registrandose.- The article is available online on the publisher's page and can be downloaded for free by the pdf registering.
Publisher version (URL)http://dx.doi.org/10.21769/BioProtoc.3474
URIhttp://hdl.handle.net/10261/197435
DOIhttp://dx.doi.org/10.21769/BioProtoc.3474.
ISSN2331-8325
Appears in Collections:(IRNAS) Artículos
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