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Título

Cloning and functional characterization of the high-affinity K+ transporter HAK1 of pepper

AutorMartínez-Cordero, M. Angeles; Martínez, Vicente ; Rubio, Francisco
Palabras clavePepper
Potassium transporters
Fecha de publicaciónoct-2004
EditorSpringer
CitaciónPlant Molecular Biology 56(3): 413-421 (2004)
ResumenHigh-affinity K+ uptake in plants plays a crucial role in K+ nutrition and different systems have been postulated to contribute to the high-affinity K+ uptake. The results presented here with pepper (Capsicum annum) demonstrate that a HAK1-type transporter greatly contributes to the high-affinity K+ uptake observed in roots. Pepper plants starved of K+ for 3 d showed high-affinity K+ uptake (K m of 6 mgrM K+) that was very sensitive to NH and their roots expressed a high-affinity K+ transporter, CaHAK1, which clusters in group I of the KT/HAK/KUP family of transporters. When expressed in yeast (Saccharomyces cerevisiae), CaHAK1 mediated high-affinity K+ and Rb+ uptake with K m values of 3.3 and 1.9 mgr M, respectively. Rb+ uptake was competitively inhibited by micromolar concentrations of NH and Cs+, and by millimolar concentrations of Na+.
Descripción9 pages, 6 figures.
Versión del editorhttp://dx.doi.org/10.1007/s11103-004-3845-4
URIhttp://hdl.handle.net/10261/19742
DOI10.1007/s11103-004-3845-4
ISSN0167-4412
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