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http://hdl.handle.net/10261/197171
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Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Sánchez-León, Susana | es_ES |
dc.contributor.author | Giménez, María J. | es_ES |
dc.contributor.author | Comino, Isabel | es_ES |
dc.contributor.author | Sousa Martín, Carolina | es_ES |
dc.contributor.author | López-Casado, Miguel Ángel | es_ES |
dc.contributor.author | Torres, María Isabel | es_ES |
dc.contributor.author | Barro Losada, Francisco | es_ES |
dc.date.accessioned | 2019-12-20T14:11:57Z | - |
dc.date.available | 2019-12-20T14:11:57Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Nutrients 11(12): 2933 (2019) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10261/197171 | - |
dc.description | © 2019 by the authors. | - |
dc.description.abstract | Wheat gluten proteins are responsible for the bread-making properties of the dough but also for triggering important gastrointestinal disorders. Celiac disease (CD) affects approximately 1% of the population in Western countries. The only treatment available is the strict avoidance of gluten in the diet. Interference RNA (RNAi) is an excellent approach for the down-regulation of genes coding for immunogenic proteins related to celiac disease, providing an alternative for the development of cereals suitable for CD patients. In the present work, we report a comparative study of the stimulatory capacity of seven low-gluten RNAi lines differing in grain gluten and non-gluten protein composition, relevant for CD and other gluten pathologies. Peripheral blood mononuclear cells (PBMCs) of 35 patients with active CD were included in this study to assess the stimulatory response induced by protein extracts from the RNAi lines. Analysis of the proliferative response and interferon-gamma (INF-γ) release of PBMCs demonstrated impaired stimulation in response to all RNAi lines. The lower response was provided by lines with a very low content of α- and γ-gliadins, and low or almost devoid of DQ2.5 and p31–43 α-gliadin epitopes. The non-gluten protein seems not to play a key role in PBMC stimulation. | - |
dc.description.sponsorship | This research was funded by the Spanish Ministry of Economy, Industry and competitiveness, grant number AGL2016-80566-P, and the European Regional Development Fund (FEDER). | - |
dc.language.iso | eng | es_ES |
dc.publisher | Multidisciplinary Digital Publishing Institute | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-80566-P | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.subject | PBMCs | - |
dc.subject | RNAi | - |
dc.subject | Low-gluten wheat | - |
dc.subject | Celiac disease | - |
dc.subject | NCWS | - |
dc.title | Stimulatory Response of Celiac Disease Peripheral Blood Mononuclear Cells Induced by RNAi Wheat Lines Differing in Grain Protein Composition | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.3390/nu11122933 | - |
dc.description.peerreviewed | Peer reviewed | - |
dc.relation.publisherversion | https://doi.org/10.3390/nu11122933 | - |
dc.identifier.e-issn | 2072-6643 | - |
dc.date.updated | 2019-12-20T14:11:57Z | - |
dc.rights.license | http://creativecommons.org/licenses/by/4.0/ | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | European Commission | - |
dc.relation.csic | Sí | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.pmid | 31816892 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.languageiso639-1 | en | - |
item.fulltext | With Fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | open | - |
item.openairetype | artículo | - |
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Stimulatory_Sanchez_Art2020.pdf | 3,27 MB | Adobe PDF | Visualizar/Abrir |
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