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The Host Factor Erlin-1 is Required for Efficient Hepatitis C Virus Infection

AuthorsWhitten-Bauer, Christina; Chung, Josan; Gómez-Moreno, Andoni; Gomollón-Zueco, Pilar; Huber, Michael D.; Gerace, Larry; Garaigorta, Urtzi
KeywordsHepatitis C virus
Host factor
Endoplasmic reticulum
RNA replication
Protein production
Virus production
Lipid droplet
Issue Date2-Dec-2019
PublisherMultidisciplinary Digital Publishing Institute
CitationCells 8(12): 1555 (2019)
AbstractDevelopment of hepatitis C virus (HCV) infection cell culture systems has permitted the identification of cellular factors that regulate the HCV life cycle. Some of these cellular factors affect steps in the viral life cycle that are tightly associated with intracellular membranes derived from the endoplasmic reticulum (ER). Here, we describe the discovery of erlin-1 protein as a cellular factor that regulates HCV infection. Erlin-1 is a cholesterol-binding protein located in detergent-resistant membranes within the ER. It is implicated in cholesterol homeostasis and the ER-associated degradation pathway. Silencing of erlin-1 protein expression by siRNA led to decreased infection efficiency characterized by reduction in intracellular RNA accumulation, HCV protein expression and virus production. Mechanistic studies revealed that erlin-1 protein is required early in the infection, downstream of cell entry and primary translation, specifically to initiate RNA replication, and later in the infection to support infectious virus production. This study identifies erlin-1 protein as an important cellular factor regulating HCV infection.
Description© 2019 by the authors.
Publisher version (URL)https://doi.org/10.3390/cells8121555
Appears in Collections:(CNB) Artículos
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