English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/197013
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Relation between serum levels of chemotaxis-related factors and the presence of coronary artery calcification as expression of subclinical atherosclerosis

AuthorsMuñoz, Juan Carlos; Martín, Rubén ; Alonso, Carmen; Gutiérrez, Beatriz; Nieto, María Luisa
KeywordsCoronary artery calcification
Atherosclerosis
Biomarkers
Netrin-1
Gremlin-1
Macrophage inflammatory protein-1β
Issue Date2017
PublisherElsevier
CitationClinical Biochemistry 50(18): 1048-1055 (2017)
Abstract[Background] Atherosclerotic plaque formation is characterized by recruitment of monocytes/macrophages, which contributes to its calcification by releasing pro-osteogenic cytokines. Chemotaxis-related proteins, including netrin-1, gremlin-1 and macrophage inflammatory protein-1β (MIP-1β), regulate immune cell migration. However, their relation with the presence of subclinical atherosclerosis, assessed by measures of coronary artery calcifications (CAC) in patients without known coronary artery disease (CAD), remains unclear.
[Aims] To examine whether these chemoattractant-related proteins are associated with the presence of CAC in patients without known CAD.
[Methods] A retrospective case-control observational study was conducted in 120 outpatients without CAD, undergoing a CAC evaluation by computed tomography with the Agatston Calcium score, categorized as CAC− (none) and CAC+ (≥ 1). Serum biomarkers were quantified by ELISA.
[Results] Lpa, dyslipidaemia and smoking were significantly higher (p = 0.006, p ≤ 0.0001 and p = 0.001, respectively) in CAC+ patients. Serum netrin-1 levels were lower in CAC+ than in CAC− patients (196.8 ± 127.8 pg/ml versus 748.3 ± 103.2 pg/ml, p ≤ 0.0001), and a similar pattern was found for gremlin-1 (1.14 ± 0.39 ng/ml versus 4.33 ± 1.20 ng/ml, p ≤ 0.0001). However, TNFα and MIP-1β were strongly upregulated in CAC+ patients (447.56 ± 74 pg/ml versus 1104 ± 144 pg/ml and 402.00 ± 94 pg/ml versus 905.0 ± 101.6 pg/ml, respectively, p ≤ 0.001). Multivariate analyses revealed that low netrin-1 and gremlin-1 levels and high TNFα and MIP-1β amounts were associated with CAC presence, after adjustment for clinical and biochemical variables.
[Conclusions] We found a netrin-1 and gremlin-1 deficiency and a TNFα and MIP-1β overproduction in CAC+ patients' serum. These proteins may be used to identify individuals with subclinical atherosclerosis. Further research is warranted in a larger cohort of patients to establish these chemotactic-related proteins as biomarkers that improve CAD risk stratification.
Publisher version (URL)https://doi.org/10.1016/j.clinbiochem.2017.08.012
URIhttp://hdl.handle.net/10261/197013
DOIhttp://dx.doi.org/10.1016/j.clinbiochem.2017.08.012
ISSN0009-9120
E-ISSN1873-2933
Appears in Collections:(IBGM) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf59,24 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.