Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/196854
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Neurotoxic Ca2+ signaling induced by amyloid–β oligomers in aged hippocampal neurons in vitro |
Autor: | Núñez, Lucía CSIC ORCID; Calvo-Rodríguez, María CSIC ORCID; García-Durillo, Mónica CSIC; Villalobos, Carlos CSIC ORCID | Palabras clave: | Alzheimer’s disease Amyloid β oligomers Hippocampal neurons Aging Calcium |
Fecha de publicación: | 2018 | Editor: | Springer Nature | Citación: | Amyloid Proteins: 341-354 (2018) | Serie: | Methods in Molecular Biology 1779 | Resumen: | Alzheimer’s disease (AD), the most prevalent dementia linked to aging, involves neurotoxic effects of amyloid β species and dishomeostasis of intracellular Ca2+. To investigate mechanisms of AD, the effects of soluble species of amyloid β oligomers (Aβo) prepared in medium devoid of glutamate receptor agonists can be tested on intracellular Ca2+ in long-term cultures of rat hippocampal neurons that reflect aging neurons. Furthermore, changes in expression of proteins involved in oligomer responses and AD can be tested in the same neurons using quantitative immunofluorescence. Detailed procedures for the preparation of Aβ species in defined medium, long-term culture of rat hippocampal neurons mimicking aged neurons, calcium imaging and quantitative immunofluorescence in these cultures are described in this chapter. | Versión del editor: | https://doi.org/10.1007/978-1-4939-7816-8_20 | URI: | http://hdl.handle.net/10261/196854 | DOI: | 10.1007/978-1-4939-7816-8_20 | ISBN: | 978-1-4939-7815-1 |
Aparece en las colecciones: | (IBGM) Libros y partes de libros |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 59,24 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
Page view(s)
174
checked on 19-abr-2024
Download(s)
23
checked on 19-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.