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dc.contributor.authorViéitez Martín, Ana Maríaes_ES
dc.contributor.authorSan José, M. Carmenes_ES
dc.contributor.authorCorredoira, Elenaes_ES
dc.date.accessioned2019-12-17T07:39:50Z-
dc.date.available2019-12-17T07:39:50Z-
dc.date.issued2010-
dc.identifier.citationPlant Embryo Culture: 201-213 (2010)es_ES
dc.identifier.isbn978-1-61737-987-1-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10261/196813-
dc.description.abstractFor Castanea sativa (European chestnut), a species with recalcitrant seeds that is not easily propagated vegetatively, cryopreservation is one of the most promising techniques for maintaining genetic resource diversity and for conservation of selected germplasms. Long-term conservation of selected seeds and valuable embryogenic lines can be achieved through the cryopreservation of zygotic embryonic axes and somatic embryos, respectively. This chapter describes methods for the desiccation-based cryostorage of zygotic embryonic axes, and the vitrification-based cryopreservation of somatic embryos. For zygotic embryonic axes, the highest post-thaw survival and plantlet recovery rates are obtained by desiccation in a laminar flow hood to 20–25% moisture content, followed by direct immersion in liquid nitrogen. For somatic embryos, embryogenesis resumption rates of over 60% are achieved by preculture of embryo clumps for 3 days on solid medium containing 0.3 M sucrose, incubation in PVS2 vitrification solution for 60 min at 0°C, and direct immersion in liquid nitrogen. Plantlet recovery from cryostored embryogenic lines requires proliferation of the thawed embryos and subsequent maturation before germination and conversion into plantlets.es_ES
dc.language.isoenges_ES
dc.publisherSpringer Naturees_ES
dc.relation.ispartofseriesMethods in Molecular Biologyes_ES
dc.relation.ispartofseries710es_ES
dc.rightsclosedAccesses_ES
dc.subjectCastanea sativaes_ES
dc.subjectChestnutses_ES
dc.subjectCryostoragees_ES
dc.subjectCryopreservationes_ES
dc.subjectEmbryo desiccationes_ES
dc.subjectEuropean chestnutes_ES
dc.subjectPlant regenerationes_ES
dc.subjectSomatic embryogenesises_ES
dc.subjectVitrificationes_ES
dc.subjectZygotic embryoses_ES
dc.titleCryopreservation of Zygotic Embryonic Axes and Somatic Embryos of European Chestnutes_ES
dc.typecapítulo de libroes_ES
dc.identifier.doihttp://dx.doi.org/10.1007/978-1-61737-988-8_15-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1007/978-1-61737-988-8_15es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.pmid21207271-
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