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http://hdl.handle.net/10261/196767
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Corredoira, Elena | es_ES |
dc.contributor.author | San José, M. Carmen | es_ES |
dc.contributor.author | Ballester, Antonio | es_ES |
dc.contributor.author | Viéitez Martín, Ana María | es_ES |
dc.date.accessioned | 2019-12-16T10:34:53Z | - |
dc.date.available | 2019-12-16T10:34:53Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | CryoLetters 25(1): 33-42 (2004) | es_ES |
dc.identifier.issn | 0143-2044 | - |
dc.identifier.uri | http://hdl.handle.net/10261/196767 | - |
dc.description.abstract | This work describes experiments demonstrating the feasibility of long-term conservation of Castanea sativa germplasm through cryopreservation of embryonic axes or somatic embryo clumps. Between 93% and 100% of excised embryonic axes of recalcitrant chestnut seeds survived storage in liquid nitrogen (LN) following desiccation in a laminar flow cabinet to moisture contents of 20-24% (on a fresh weight basis), and some 63% subsequently developed as whole plants. Desiccation to moisture contents less than 19% produced damage resulting in loss of organized plant development after cryostorage, allowing only root growth. When 6-8 mg clumps of globular or heart-shaped somatic embryos were precultured for 7 days on high-sucrose medium and then desiccated to a moisture content of 25% before storage in LN, the embryogenesis resumption level after thawing was 33%. When the embryo clumps were precultured for 3 days on high-sucrose medium followed by 60 min application of PVS2 vitrification solution before cryostorage, the post-storage embryogenesis resumption level was 68%. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Cryoletters | es_ES |
dc.rights | closedAccess | es_ES |
dc.title | Cryopreservation of zygotic embryo axes and somatic embryos of European chestnut | es_ES |
dc.type | artículo | es_ES |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.pmid | 15031743 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
item.openairetype | artículo | - |
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accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
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