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Title

Cryopreservation of zygotic embryo axes and somatic embryos of European chestnut

AuthorsCorredoira, Elena ; San José, M. Carmen; Ballester, Antonio ; Viéitez Martín, Ana María
Issue Date2004
PublisherCryoletters
CitationCryoLetters 25(1): 33-42 (2004)
AbstractThis work describes experiments demonstrating the feasibility of long-term conservation of Castanea sativa germplasm through cryopreservation of embryonic axes or somatic embryo clumps. Between 93% and 100% of excised embryonic axes of recalcitrant chestnut seeds survived storage in liquid nitrogen (LN) following desiccation in a laminar flow cabinet to moisture contents of 20-24% (on a fresh weight basis), and some 63% subsequently developed as whole plants. Desiccation to moisture contents less than 19% produced damage resulting in loss of organized plant development after cryostorage, allowing only root growth. When 6-8 mg clumps of globular or heart-shaped somatic embryos were precultured for 7 days on high-sucrose medium and then desiccated to a moisture content of 25% before storage in LN, the embryogenesis resumption level after thawing was 33%. When the embryo clumps were precultured for 3 days on high-sucrose medium followed by 60 min application of PVS2 vitrification solution before cryostorage, the post-storage embryogenesis resumption level was 68%.
URIhttp://hdl.handle.net/10261/196767
ISSN0143-2044
Appears in Collections:(IIAG) Artículos
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