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Improving chestnut micropropagation through axillary shoot development and somatic embryogenesis

AuthorsBallester, Antonio ; Bourrain, Laurence; Corredoira, Elena ; Gonçalves, José Carlos; Lê, Công-Linh; Miranda -Fontaíña, María Eugenia; San José, M. Carmen; Sauer, Ursula; Viéitez Martín, Ana María
KeywordsAxillary shoots
Castanea sativa
C. sativa x C. crenata
Somatic embryogenesis
Issue DateJan-2001
PublisherSwiss Federal Institute for Forest, Snow, and Landscape Research
CitationForest Snow and Landscape Research 76(3):460-467 (2001)
AbstractThe effects of the mineral media and the carbon source on the proliferation capacity of different Castanea sativa x C. crenata cultivars were the focus of the research reported here. Using the com-mercial cultivar Marigoule, the addition of riboflavin to the rooting medium did not improve the rooting rates recorded. It did, however, positively affect the survival of regenerated plantlets after weaning. By measuring certain physiological parameters, the beneficial effect of high concen -trations of CO 2 on the acclimatization of chestnut regenerated plantlets was recorded. However, general protocols for large-scale micropropagation of specific cultivars could not be defined. Our research has determined for the first time the developmental window in which somatic embryogenesis induction is possible from ovaries, ovules and/or zygotic embryos in C. sativa. Induction is possible between the 2 nd and the l0 th weeks post-anthesis, giving an overall frequency of 4.5%. Somatic embryogenesis induction in chestnut was not possible from mature tissues; however, embryogenesis was achieved using leaf tissue from shoot multiplication cultures. This indicates, for the first time, that material from explants other than zygotic chestnut embryos is com petent for somatic embryogenesis. The effect of thidiazuron on the ability of different seedling explants of chestnut to induce multiple shoots was also evaluated: cotyledonary node explants, which contain preformed meris-tematic tissue, were the only responsive explants.
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