Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/196647
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dc.contributor.authorEcheverría, Silvinaes_ES
dc.contributor.authorLeiguez, Elbioes_ES
dc.contributor.authorGuijas, Carloses_ES
dc.contributor.authorGalvão do Nascimento, Neidees_ES
dc.contributor.authorAcosta, Ofeliaes_ES
dc.contributor.authorTeixeira, Catarinaes_ES
dc.contributor.authorLeiva, Laura C.es_ES
dc.contributor.authorRodríguez, Juan Pabloes_ES
dc.date.accessioned2019-12-13T10:22:20Z-
dc.date.available2019-12-13T10:22:20Z-
dc.date.issued2018-
dc.identifier.citationChemico-Biological Interactions 281: 24-31 (2018)es_ES
dc.identifier.issn0009-2797-
dc.identifier.urihttp://hdl.handle.net/10261/196647-
dc.description.abstractInflammation is a major local feature of envenomation by bothropic snakes being characterized by a prominent local edema, pain, and extensive swelling. There are reports demonstrating that whole Bothrops snake venoms and toxins isolated from them are able to activate macrophages functions, such as phagocytosis, production of reactive oxygen, cytokines and eicosanoids, however, little is known about the effects of Bothrops alternatus (B.a.) venom on macrophages. In this work, we evaluated the proinflammatory effects of B.a. venom with in vivo and in vitro experiments using the Raw 264.7 cell line and mouse peritoneal macrophages. We detected that B.a. venom augments cell permeability (2-fold), and cellular extravasation (mainly neutrophils), increase proinflammatory cytokines IL1 (∼300-fold), IL12 (∼200-fold), and TNFα (∼80-fold) liberation and induce the expression of enzymes related to lipid signaling, such as cPLA2α and COX-2. Additionally, using lipidomic techniques we detected that this venom produces a release of arachidonic acid (∼10 nMol/mg. Protein) and other fatty acids (16:0 and 18:1 n-9c). Although much of these findings were described in inflammatory processes induced by other bothropic venoms, here we demonstrate that B.a. venom also stimulates pro-inflammatory pathways involving lipid mediators of cell signaling. In this sense, lipidomics analysis of macrophages stimulated with B.a. venom evidenced that the main free fatty acids are implicated in the inflammatory response, and also demonstrated that this venom, is able to activate lipid metabolism even with a low content of PLA2.es_ES
dc.description.sponsorshipThis research was financial supported by Secretaría General de Ciencia y Técnica (SGCyT) CF01/2013 Universidad Nacional del Nordeste (UNNE).es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsclosedAccesses_ES
dc.subjectImmunotoxicologyes_ES
dc.subjectInflammationes_ES
dc.subjectMyotoxines_ES
dc.subjectPLA2es_ES
dc.subjectAntivenomses_ES
dc.subjectLipidomicses_ES
dc.titleEvaluation of pro-inflammatory events induced by Bothrops alternatus snake venomes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.cbi.2017.12.022-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.cbi.2017.12.022es_ES
dc.identifier.e-issn1872-7786-
dc.contributor.funderSecretaría de Ciencia y Técnica de la Nación (Argentina)es_ES
dc.contributor.funderUniversidad Nacional del Nordeste (Argentina)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100010325es_ES
dc.identifier.pmid29248447-
item.fulltextNo Fulltext-
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
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