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Title

Etiolation as a pretreatment for in vitro establishment and multiplication of mature chestnut

AuthorsBallester, Antonio ; Sánchez Fernández, M.ª Concepción; Viéitez Martín, Ana María
KeywordsCastanea
Chestnut
Etiolation
In vitro tissue culture
Issue DateNov-1989
PublisherJohn Wiley & Sons
CitationPhysiologia Plantarum 77(3): 395-400 (1989)
AbstractLocalized etiolation of branches in the crown of a 30‐year‐old chestnut tree produced plant material that responded much better to establishment and multiplication in vitro than unetiolated material, whose cultures were very difficult to maintain (response being measured in terms of the percentage of cultures established, the mean number of shoots formed per explant, the number of 8‐mm segments per new shoot, the length of longest shoot in each culture, and the coefficient of multiplication). Only 22% of the initial explants from unetiolated material were successfully established, as against 79% for etiolated material, with similar differences between the coefficients of multiplication of the two lines in successive cultures. Accordingly, partial etiolation of branches is proposed as a suitable pretreatment for in vitro propagation of selected mature trees, when physiologically juvenile materials such as stump sprouts, epicormic shoots or root suckers are not available.
Publisher version (URL)https://doi.org/10.1111/j.1399-3054.1989.tb05659.x
URIhttp://hdl.handle.net/10261/196367
DOIhttp://dx.doi.org/10.1111/j.1399-3054.1989.tb05659.x
ISSN0031-9317
E-ISSN1399-3054
Appears in Collections:(IIAG) Artículos
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