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Voltammetry assisted by multivariate analysis as a tool for speciation of metallothioneins: competitive complexation of α- and β-metallothionein domains with cadmium and zinc

AuthorsLópez, María José; Ariño, Cristina; Díaz-Cruz, M. Silvia ; Díaz-Cruz, José Manuel; Tauler, Romà ; Esteban, Miquel
KeywordsMultivariate Curve Resolution by Alternating Least Squares (MCR-ALS)
β-domains of Metallothionein (MT)
α-domains of Metallothionein (MT)
Issue Date4-Nov-2003
PublisherAmerican Chemical Society
CitationEnvironmental Science and Technology 37(24): 5609–5616 (2003)
AbstractMultivariate Curve Resolution by Alternating Least Squares (MCR-ALS) is applied to voltammetric data obtained in the study of competitive complexation of Zn(II) and Cd(II) by α- and β-domains of metallothionein (MT). The application of MCR-ALS allows the estimation of both the voltammograms and the concentration profiles associated with each electrochemical process. The complexity of the voltammograms obtained in titrations of the α- or β-domains of MT with Cd(II) and later Zn(II) (or vice versa) prevents their direct interpretation using traditional electrochemical methods. But when MCR-ALS is applied, voltammograms can be interpreted rather satisfactorily in both qualitative and quantitative terms. MCR-ALS showed the formation of Cd2Znβdom and Cd3Znαdom complexes when both metals were competitively added. A method based on the combined use of voltammetry and some chemometric techniques is proposed. It can be useful for the metal speciation of environmentally relevant natural ligands.
Description8 pages, 8 figures, 1 table.-- PMID: 14717171 [PubMed].-- Printed version published Dec 15, 2003.
Publisher version (URL)http://dx.doi.org/10.1021/es030048n
Appears in Collections:(IDAEA) Artículos
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