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Title

Effect of orange by-products on inflammatory indicators in a DSS mouse model

AuthorsPacheco, M. Teresa; Vezza, Teresa; Diez-Echave, Patricia; Utrilla, Pilar M.; Villamiel, Mar CSIC ORCID ; Moreno, F. Javier CSIC ORCID
Issue Date2018
CitationXII Congreso Internacional Nutrición, Alimentación y Dietética (2018)
Abstract[Introducción] Inflammation is a response triggered by damage to living tissues. The mechanisms involved in the initiation and perpetuation of inflammatory bowel disease (IBD) are not known exactly; however, there are mediators released in the early stages and may act as indicators of the disease. Colonic cytokines production and macroscopic indicators such as weight loss, reduction of food consumption, soft stools, among others, allow to evaluate the degree of IBD. Orange by-products (OBP) namely: fresh residue (FOR), dry residue (DOR), liquor (OL) and animal feed (AF), analysed in a previous study contain: phenols, pectin and/or furosine (Maillard reaction product). These compounds can show anti-oxidant, anti-bacterial and anti-cancer effect, but its anti-inflammatory activity is still little studied.
[Objetivos] In consequence the aim of this work was to study the effect of OBP ingestion on IBD, using a DSS mice model; which exhibits similar characteristics to those present in human Crohn´s disease.
[Métodos] 2-Furoylmethyl-Amino Acids (2-FM-AA) were analyzed by ionpair RP-HPLC-UV, and monosaccharides through hydrolysis with 2 M trifluoroacetic acid and subsequent formation of trimethylsilyloximes derivatives, by GC-FID. Male C57BL/6 mice (Janvier, St Berthevin Cedex, France) were distributed in 7 groups: healthy, DSS control, FOR, DOR, OL, AF and Citrus pectin (CP) (CEAMSA, O Porriño, Spain) was used as standard. Animals pre-treatment start 15 days before induction of colitis with DSS (2,7%) on drinking water. On day 22, animals were euthanized and colon samples were obtained. Macroscopic indicators were observed every day (body weigth, food consumption, disease activity index (DAI)), and tissue pro-inflammatory cytoquines and intestinal protective proteins were determined by Real-Time quantitative PCR (EcoTM Real-Time PCR System, Illumina, CA, USA).
[Resultados] Weights lost was high for DSS control group and CP, AF and FOR significantly reduced the drop. In addition, FOR and CP groups, presented low disease activity index (DAI) values vs. DSS control group (p < 0.05). On the contrary, DOR and OL groups showed a DAI value similar to non-treated groups. Colonic weight/length ratio was significantly less in all the OBP or CP treated mice vs the DSS group. Colonic inflammatory cytokines expression as TNF-α, IL-1β, IL-6 and NOS were significantly reduced in AF and CP group and expression of protein with intestinal barrier functions as ICAM I, MUC 3, occludin, and ZO-1 were also positively modified in mice treated with FOR, AF and CP (p < 0.05).
[Discusión] Analyzing the consumption effect of AF vs. CP; CP intake allowed to obtain, a better anti-inflammatory response, which could be due to its greatest content of soluble dietary fiber SDF and N-fructosyl-lysine.
[Conclusiones] In general, CP and AF intake allowed to obtain a better anti-inflammatory response in DSS model of IBD compared with other OBP products assayed, which could be due to its greatest content of furosine. The product namely FOR also show a beneficial effect in some parameters measured probably due at its biggest phenols content. The evaluated indicators underline the potential role of pectin and Maillard reaction compounds in ameliorating some IBD symptoms. However, in order to reach more accurate conclusions, the analysis of additional biochemical indicators should be conducted.
DescriptionTrabajo presentado al XII Congreso Internacional Nutrición, Alimentación y Dietética, celebrado en Madrid el 11 y 12 de abril de 2018.
URIhttp://hdl.handle.net/10261/195118
Appears in Collections:(CIAL) Comunicaciones congresos

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