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A simple strategy for improving bird sexing from highly degraded DNA samples

AuthorsMorinha, Francisco; Bautista, Luis M. ; Monteiro, Marlene; Alonso López, Juan C.
Sex-linked markers
Molecular sexing
Degraded DNA
Real-time PCR
Grus grus
Issue DateDec-2019
PublisherSpringer Nature
CitationConservation Genetics Resources 11(4): 393-396 (2019)
AbstractA simple methodology was developed to select new sex-specific primers for bird sexing from degraded and low-quantity DNA sources. The strategy was validated using highly degraded DNA extracted from Giemsa-stained blood smears of common cranes (Grus grus). The new primers allowed the accurate molecular sexing using (i) a classic approach of PCR followed by agarose gel electrophoresis and (ii) an advanced real-time PCR method. The simplicity, speed and low cost make this methodology a versatile molecular tool for selection of novel markers/primers for bird sex differentiation from complex DNA sources, which can be used as basis or complement in several fields of ornithological research.
Publisher version (URL)https://doi.org/10.1007/s12686-018-1030-3
Appears in Collections:(MNCN) Artículos
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