English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/193977
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Early transcriptional response to biotic stress in mixed starter fermentations involving Saccharomyces cerevisiae and Torulaspora delbrueckii

AuthorsTronchoni, Jordi ; Curiel, José Antonio ; Morales, Pilar ; Torres-Pérez, Rafael; González García, Ramón
Issue Date26-Jun-2017
Citation33rd International Specialised Symposium on Yeast (2017)
7th Congress of European Microbiologists FEMS (2017)
AbstractAdvances in microbial wine biotechnology have led to the recent commercialization of several non-Saccharomyces starter cultures. These are intended to be used in either simultaneous or sequential inoculation with Saccharomyces cerevisiae. We analysed the transcriptional response to co-cultivation of S. cerevisiae and Torulaspora delbrueckii. The study is focused in the initial stages of wine fermentation with the goal of better understand the microbial interactions that can be stablished during wine fermentation with mixed-starters. Fermentations were carried out in bioreactors using synthetic grape must to mimic industrial conditions. Experiments were carried out in triplicate for fermentation kinetics, CO2 performance and RNAseq analysis. Both species showed a clear response to the presence of each other, even though the portion of the genome showing altered transcriptional levels was relatively small. Changes in the transcription pattern suggested a stimulation of metabolic activity and growth, as a consequence of the presence of competitors in the same medium. The response of S. cerevisiae seems to take place earlier, as compared to T. delbrueckii. Enhanced glycolytic activity of the mixed culture was confirmed by the CO2 production profile during these early stages of fermentation. Interestingly, HSP12 expression appeared induced by co-cultivation for both of S. cerevisiae and Torulaspora delbrueckii in the two time points studied. This might be related with a recently described role of Hsp12 in intercellular communication in yeast. Expression of S. cerevisiae PAU genes was also stimulated in mixed cultures.
DescriptionTrabajo presentado en el 33rd International Specialised Symposium on Yeast (ISSY33), celebrado en Cork (Irlanda), del 26 al 29 de junio de 2017 -- Trabajo presentado al 7th Congress of European Microbiologists (FEMS), celebrado en Valencia (España) del 9 al 13 de julio de 2017
URIhttp://hdl.handle.net/10261/193977
Appears in Collections:(ICVV) Comunicaciones congresos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,35 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.