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dc.contributor.authorMorales, Diegoes_ES
dc.contributor.authorRibeiro Smiderle, Fhernandaes_ES
dc.contributor.authorVillalva, Marisoles_ES
dc.contributor.authorAbreu, Hellenes_ES
dc.contributor.authorRico, Claudiaes_ES
dc.contributor.authorSantoyo, Susanaes_ES
dc.contributor.authorIacomini, Marcelloes_ES
dc.contributor.authorSoler-Rivas, Cristinaes_ES
dc.date.accessioned2019-10-30T10:57:02Z-
dc.date.available2019-10-30T10:57:02Z-
dc.date.issued2019-
dc.identifier.citationJournal of Functional Foods 60: 103446 (2019)es_ES
dc.identifier.issn1756-4646-
dc.identifier.urihttp://hdl.handle.net/10261/193796-
dc.description.abstractInnovative technologies as ultrasound-assisted extraction (UAE) (550 W, 60% amplitude, 50 °C) or subcritical water extraction (SWE) (200 °C, 11.7 MPa) were more effective than hot water extractions to obtain β-glucan-enriched fractions from shiitake mushrooms. UAE required longer extraction time (60 min) than SWE (15 min). Combination of UAE + SWE or pre-treatment of the raw material with supercritical CO2 (SFE) (40 °C, 35 MPa, 3 h) before both extractions yielded extracts containing larger β-glucan concentrations. Fluorimetric/colorimetric determinations indicated that obtained fractions contained (1 → 3)- and (1 → 3),(1 → 6)-β-glucans. NMR confirmed their presence as well as (1 → 3)-α-glucans and heteropolymers including mannose and galactose. SWE (15 min), SFE + SWE or UAE + SWE extracts showed larger glucose levels and lower mannose and galactose residues than the other extractions suggesting certain extraction specificity towards β-glucans. They also included more chitin-derivatives than UAE. The extracts obtained after combination of technologies partially retained their immunomodulatory properties but they showed high hypocholesterolemic activities according to in vitro studies.es_ES
dc.description.sponsorshipThis research was supported by national R+ D program from the Spanish Ministry of Science and Innovation (project AGL2014-56211-R) and the regional program from the Community of Madrid, Spain (S2013/ABI-2728).es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationMINECO/ICTI2013-2016/AGL2014-56211-Res_ES
dc.relationS2013/ABI-2728/ALIBIRDes_ES
dc.relation.isversionofPostprint-
dc.rightsembargoedAccesses_ES
dc.subjectβ-Glucanes_ES
dc.subjectShiitake mushroomses_ES
dc.subjectUltrasound-assisted extractiones_ES
dc.subjectSubcritical water extractiones_ES
dc.subjectHypocholesterolemic activityes_ES
dc.subjectImmunomodulatory activityes_ES
dc.titleTesting the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodeses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.jff.2019.103446-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.jff.2019.103446es_ES
dc.identifier.e-issn2214-9414-
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderComunidad de Madrides_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/100012818es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004837es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100012818es_ES
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