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Testing the effect of combining innovative extraction technologies on the biological activities of obtained β-glucan-enriched fractions from Lentinula edodes

AuthorsMorales, Diego ; Ribeiro Smiderle, Fhernanda; Villalva, Marisol ; Abreu, Hellen; Rico, Claudia; Santoyo, Susana ; Iacomini, Marcello; Soler-Rivas, Cristina
Shiitake mushrooms
Ultrasound-assisted extraction
Subcritical water extraction
Hypocholesterolemic activity
Immunomodulatory activity
Issue Date2019
CitationJournal of Functional Foods 60: 103446 (2019)
AbstractInnovative technologies as ultrasound-assisted extraction (UAE) (550 W, 60% amplitude, 50 °C) or subcritical water extraction (SWE) (200 °C, 11.7 MPa) were more effective than hot water extractions to obtain β-glucan-enriched fractions from shiitake mushrooms. UAE required longer extraction time (60 min) than SWE (15 min). Combination of UAE + SWE or pre-treatment of the raw material with supercritical CO2 (SFE) (40 °C, 35 MPa, 3 h) before both extractions yielded extracts containing larger β-glucan concentrations. Fluorimetric/colorimetric determinations indicated that obtained fractions contained (1 → 3)- and (1 → 3),(1 → 6)-β-glucans. NMR confirmed their presence as well as (1 → 3)-α-glucans and heteropolymers including mannose and galactose. SWE (15 min), SFE + SWE or UAE + SWE extracts showed larger glucose levels and lower mannose and galactose residues than the other extractions suggesting certain extraction specificity towards β-glucans. They also included more chitin-derivatives than UAE. The extracts obtained after combination of technologies partially retained their immunomodulatory properties but they showed high hypocholesterolemic activities according to in vitro studies.
Publisher version (URL)https://doi.org/10.1016/j.jff.2019.103446
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