Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/193520
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

Determination of ligand profiles for pseudomonas aeruginosa solute binding proteins

AuthorsFernández Rodríguez, Matilde ; Rico-Jiménez, Miriam; Ortega, Álvaro; Daddaoua, Abdelali; García García, Ana Isabel; Martín-Mora, David; Mesa Torres, Noel; Tajuelo, Ana; Matilla, Miguel A.; Krell, Tino
KeywordsSolute binding protein
Transport
Chemotaxis
Ligand recognition
Issue Date17-Oct-2019
PublisherMultidisciplinary Digital Publishing Institute
CitationInternational Journal of Molecular Sciences 20(20): 5156 (2019)
AbstractSolute binding proteins (SBPs) form a heterogeneous protein family that is found in all kingdoms of life. In bacteria, the ligand-loaded forms bind to transmembrane transporters providing the substrate. We present here the SBP repertoire of Pseudomonas aeruginosa PAO1 that is composed of 98 proteins. Bioinformatic predictions indicate that many of these proteins have a redundant ligand profile such as 27 SBPs for proteinogenic amino acids, 13 proteins for spermidine/putrescine, or 9 proteins for quaternary amines. To assess the precision of these bioinformatic predictions, we have purified 17 SBPs that were subsequently submitted to high-throughput ligand screening approaches followed by isothermal titration calorimetry studies, resulting in the identification of ligands for 15 of them. Experimentation revealed that PA0222 was specific for γ-aminobutyrate (GABA), DppA2 for tripeptides, DppA3 for dipeptides, CysP for thiosulphate, OpuCC for betaine, and AotJ for arginine. Furthermore, RbsB bound D-ribose and D-allose, ModA bound molybdate, tungstate, and chromate, whereas AatJ recognized aspartate and glutamate. The majority of experimentally identified ligands were found to be chemoattractants. Data show that the ligand class recognized by SPBs can be predicted with confidence using bioinformatic methods, but experimental work is necessary to identify the precise ligand profile.
Publisher version (URL)https://doi.org/10.3390/ijms20205156
URIhttp://hdl.handle.net/10261/193520
DOI10.3390/ijms20205156
E-ISSN1422-0067
Appears in Collections:(EEZ) Artículos




Files in This Item:
Show full item record
Review this work

PubMed Central
Citations

6
checked on May 16, 2022

SCOPUSTM   
Citations

12
checked on May 17, 2022

WEB OF SCIENCETM
Citations

12
checked on May 23, 2022

Page view(s)

215
checked on May 23, 2022

Download(s)

194
checked on May 23, 2022

Google ScholarTM

Check

Altmetric

Dimensions


Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.