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Pushing the limits of 18S rDNA variants: A temporal and spatial study on marine heterotrophic flagellates

AuthorsMassana, Ramon ; Obiol, Aleix
Issue Date30-Jul-2018
Citation5th Joint Meeting of the Phycological Society of America & International Society of Protistologists (2018)
AbstractStudies on microbial diversity have greatly advanced by high throughput sequencing of phylogenetic gene markers retrieved from natural assemblages. Now, it is feasible to simultaneously obtain thousands of sequences from hundreds of samples, and one of the main challenges is to discern very-similar real variants from the numerous low-frequency generated errors. Our main aim here is to identify the relevant variability within marine heterotrophic flagellates, a functional group of small bacterivorous protists including many uncultured species, and survey these variants along extensive temporal and spatial datasets. We use metabarcoding data from the V4 region of the 18S rDNA targeting marine picoeukaryotes, which were sampled at the global Malaspina expedition and the Blanes Bay Microbial Observatory (monthly during 10 years). By comparing different clustering methodologies, we identify single-nucleotide variants with contrasted temporal and spatial distributions. The combination of extensive sampling efforts and powerful sequencing and analytic tools enables us, for the first time, to conduct a global study to identify the dominant heterotrophic flagellates and better define their prevalence and relevance in the oceans.
Description5th Joint Meeting of the Phycological Society of America (PSA) & International Society of Protistologists Annual Meeting (ISOP), 29 July - 2 August 2018, Vancouver, BC, Canada
Appears in Collections:(ICM) Comunicaciones congresos
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