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Improvement of protocols that increase sperm production in the flatfish Solea senegalensis by using recombinant gonadotropins

AuthorsChauvigné, François ; González, Wendy; Ramos, Sandra; Ducat, Carla; Duncan, Neil; Giménez, Ignacio; Cerdà, Joan
Issue Date3-Jun-2018
Citation11th International Symposium on Reproductive Physiology of Fish : 11 ISRPF 2018 : Program and abstracts: 127 (2019)
AbstractIntroduction. Sperm production of captive Senegalese sole, Solea senegalensis, remains a major hindrance to commercial production of this highly valued species. New biotechnological approaches involving efficient hormonal therapies that increase sperm production are thus needed to establish manageable in vitro fertilization protocols. Homologous recombinant gonadotropins, follicle stimulating and luteinizing hormones (rFsh and rLh, respectively), produced in mammalian host cells, have recently arisen as promising candidates. Previous trials on juvenile fish using these hormones indicated that treatment with rFsh stimulates spermatogenesis, whereas rLh potentiates spermatozoa differentiation. The aim of the present work was to set up a protocol using rFsh and rLh to enhance sperm production in adult sole males. Material and methods. Senegalese sole males (~1 kg) were injected with rFsh (9 or 18 μg/kg) each week for 5 weeks, and on the 6th week treated with a single injection of rLh (9 or 18 μg/kg). Twenty-four hours after rLh injection, gonadotropin and androgen levels were determined by enzyme-linked immunosorbent assay (ELISA), and semen quantity and quality were evaluated by computer-assisted sperm analysis (CASA). Trials were carried out in early spring (temperatures from 13ºC to 17ºC), and in autumn under a constant temperature of 12°C. Results and Discussion. Treatment of males with rFsh and rLh during spring was not effective at inducing sperm production, possibly because of an advanced stage of sexual maturation of the males, as reflected by the high basal plasma levels of Lh (17 ng/ml). However, under constant low temperature in autumn, when circulating Lh is much lower (3 ng/ml), the dose of 9 ug/kg rFsh and rLh generated a four-fold increase in sperm production, which was not observed with the 18 ug/kg dose. It seems likely that, as previously found in juvenile fish, sustained treatment with high doses of rFsh diminished Leydig cell survival. Nevertheless, the motility, progressivity and velocity of spermatozoa were enhanced after rLh treatment during both spring and autumn. Conclusion. The data indicate that combined treatments with rFsh and rLh increase sperm production and quality in Senegalese sole adult males. However, the effectiveness of these hormonal therapies appears to be highly dependent on the reproductive stage of the males, and the temperature during which rFsh-induced spermatogenesis takes place
Description11th International Symposium on Reproductive Physiology of Fish (ISRPF-2018), New frontiers in reproductive diversity in a changing environment, 3-8 June 2018, Manaus, Brazil.-- 1 page
Appears in Collections:(ICM) Comunicaciones congresos
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