Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/192536
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Exploiting xylan as sugar donor for the synthesis of an antiproliferative xyloside using an enzyme cascade

AutorNieto-Domínguez, Manuel José CSIC ORCID; Martínez-Fernández, José Alberto; Fernández de Toro, Beatriz CSIC ; Méndez-Líter, Juan A. CSIC ; Cañada, F. Javier ; Prieto Orzanco, Alicia CSIC ORCID ; Eugenio, Laura I. de ; Martínez, María Jesús CSIC ORCID
Palabras claveEndoxylanase
β-Xylosidase
Response surface methodology
Transxylosylation
Antiproliferative
Fecha de publicación10-oct-2019
EditorBioMed Central
CitaciónMicrobial Cell Factories 18(1): 174 (2019)
Resumen[Background] Currently, industrial societies are seeking for green alternatives to conventional chemical synthesis. This demand has merged with the efforts to convert lignocellulosic biomass into value-added products. In this context, xylan, as one of main components of lignocellulose, has emerged as a raw material with high potential for advancing towards a sustainable economy.
[Results] In this study, the recombinant endoxylanase rXynM from the ascomycete Talaromyces amestolkiae has been heterologously expressed in Pichia pastoris and used as one of the catalysts of an enzyme cascade developed to synthesize the antiproliferative 2-(6-hydroxynaphthyl) β-d-xylopyranoside, by transglycosylation of 2,6-dihydroxynaphthalene. The approach combines the use of two fungal xylanolytic enzymes, rXynM and the β-xylosidase rBxTW1 from the same fungus, with the cost-effective substrate xylan. The reaction conditions for the cascade were optimized by a Central Composite Design. Maximal productions of 0.59 and 0.38 g/L were reached using beechwood xylan and birchwood xylan, respectively. For comparison, xylans from other sources were tested in the same reaction, suggesting that a specific optimization is required for each xylan variety. The results obtained using this enzyme cascade and xylan were similar or better to those previously reported for a single catalyst and xylobiose, an expensive sugar donor.
[Conclusions] Beechwood and birchwood xylan, two polysaccharides easily available from biomass, were used in a novel enzyme cascade to synthetize an antiproliferative agent. The approach represents a green alternative to the conventional chemical synthesis of 2-(6-hydroxynaphthyl) β-d-xylopyranoside using a cost-effective substrate. The work highlights the role of xylan as a raw material for producing value-added products and the potential of fungal xylanolytic enzymes in the biomass conversion.
Versión del editorhttps://doi.org/10.1186/s12934-019-1223-9
URIhttp://hdl.handle.net/10261/192536
DOI10.1186/s12934-019-1223-9
E-ISSN1475-2859
Aparece en las colecciones: (CIB) Artículos




Ficheros en este ítem:
Mostrar el registro completo

CORE Recommender

PubMed Central
Citations

3
checked on 23-mar-2024

SCOPUSTM   
Citations

9
checked on 28-mar-2024

WEB OF SCIENCETM
Citations

8
checked on 25-feb-2024

Page view(s)

229
checked on 28-mar-2024

Download(s)

157
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


Este item está licenciado bajo una Licencia Creative Commons Creative Commons